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. 2015 Sep 11;201(3):937–949. doi: 10.1534/genetics.115.181479

Figure 3.

Figure 3

Rad53 hypersignaling in cells lacking PPH3 and/or SLX4 converges to misregulation of the Mus81-Mms4 endonuclease. (A and B) Serial dilution assay showing the effect of MUS81 or SGS1 deletion on the MMS sensitivity of the indicated strains. (C–E) Effect of the rad53-R605A allele on (C) MMS sensitivity, (D) S-phase progression, and (E) PFGE-monitored chromosomes of the indicated strains lacking MUS81. In D, cells were released from G1 arrest in YPD medium containing 0.015% MMS. In E, asynchronous cells were treated with 0.01% MMS for 2 hr and released into MMS-free medium for up to 5 hr. Samples were taken at each indicated time point. (F) Serial dilution assay showing the effect of SGS1 deletion on the MMS sensitivity of a pph3Δ slx4-S486A strain.