Figure 4.

Antagonistic roles for γ-H2A in DDC control. (A and B) Effect of an H2A phospho-mutant (hta-S129A) on the MMS sensitivity (A) and Rad53 phosphorylation (B) of slx4Δ and pph3Δ strains. In B, asynchronous cells were treated with 0.005% MMS for 2 hr and samples were collected. Western blot analyses were performed using anti-Rad53 and anti-γ-H2A antibodies as described in Material and Methods. (C) Serial dilution assay showing the effect of DOT1 deletion on the MMS sensitivity of slx4Δ and pph3Δ strains. (D) Proposed model conciliating the antagonistic roles of γ-H2A on DDC regulation.