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. Author manuscript; available in PMC: 2016 Apr 15.
Published in final edited form as: Nature. 2015 Oct 15;526(7573):385–390. doi: 10.1038/nature15700

Extended Data Figure 4. AMso plasticity is regulated by AMso genetic sex.

Extended Data Figure 4

a, Diagram of the left AMso and MCM lineage.

b and c, proportion of individuals with MCMs in control animals and animals expressing sex-reversing transgenes in AMso. b, AMso masculinization with grl-2prom::fem-3::SL2::mCherry transgenes (oleEx18 and oleEx24). c, AMso feminization with grl-2prom::tra-2IC::SL2::mCherry transgenes (oleEx19 and oleEx23) and ztf-16enhancer::tra-2IC::SL2::mCherry transgene oleEx22. MCM cell fate was identified with ida-1prom::gfp or rab-3prom::yfp reporter transgenes. In the head, the grl-2 promoter drives expression in AMso and the excretory duct and pore cells, and the ztf-16 glial enhancer drives expression in the AMso and amphid sheath glia. # indicates an independent transgenic array line for each manipulation. χ2 test was used for statistical analysis; ***P<0.001; n.s.= no statistical significant difference (p≥0.05); n= number of animals scored.