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. Author manuscript; available in PMC: 2016 May 18.
Published in final edited form as: Nature. 2015 Oct 28;527(7578):371–374. doi: 10.1038/nature15758

Extended Data Figure 5. Perturbation of Notch signaling does not alter Neurogenin expression or bipolar tail neuron specification and differentiation.

Extended Data Figure 5

a, Upper panel, lateral view of a stage 23 embryo electroporated with Msx>H2B∷mCherry (magenta nuclei) and Neurogenin (Neurog) b-line>unc-76∷eGFP (green) and Msx>nls∷lacZ, serving as the wild-type “control” condition. Lower panel, embryo electroporated with same reporters as upper panel, plus Msx>Su(H)-DBM, which encodes a DNA-binding mutant form of the Notch co-activator Rbpj. No discernable difference in Neurog activation or bipolar tail neuron (BTN) specification was observed between control and Su(H)-DBM conditions (1/32 vs. 2/42 embryos showing ectopic Neurog+ BTNs, respectively). b, Late overexpression of Su(H)-DBM using the Neurog b-line driver similarly did not alter BTN specification/differentiation, as monitored by Asic>unc-76∷eGFP reporter expression (0/50 control vs. 0/50 Su(H)-DBM embryos showed ectopic Asic+ BTNs). All scale bars 50 μm.