Figure 4.

Sensitivity of human ovarian carcinoma cells to nutlin-3a is altered by the presence of endogenous and exogenous WT TP53. (A) Western blot of lysates from human cell lines treated with nutlin-3a at the IC₅₀ for each cell line. (B) TP53 activity (luciferase assay) was measured in lysates of cells treated with DMSO or nutlin-3a at their predetermined IC₅₀. (C) Western blots of GFP-tagged transfected WT TP53 and endogenous TP53 in cells treated with DMSO or nutlin-3a at the IC₅₀ for each cell line. (D) Viability of cells transfected with empty vector or WT TP53. All assays were run at least three times; Western blots are representative of three experiments.