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. 1993 Jun 1;90(11):4912–4916. doi: 10.1073/pnas.90.11.4912

Colocalization of centromeric and replicative functions on autonomously replicating sequences isolated from the yeast Yarrowia lipolytica.

P Fournier 1, A Abbas 1, M Chasles 1, B Kudla 1, D M Ogrydziak 1, D Yaver 1, J W Xuan 1, A Peito 1, A M Ribet 1, C Feynerol 1, et al.
PMCID: PMC46623  PMID: 8506336

Abstract

Two sequences (ARS18 and ARS68) displaying autonomous replication activity were previously cloned in the yeast Yarrowia lipolytica. The smallest fragment (1-1.3 kb) required for extrachromosomal replication of a plasmid is significantly larger in Y. lipolytica than in Saccharomyces cerevisiae. Neither autonomously replicating sequence (ARS) is homologous with known ARS or centromere (CEN) consensus sequences. They share short regions of sequence similarity with each other. These ARS fragments also contain Y. lipolytica centromeres: (i) integration of marker genes at the ARS loci results in a CEN-linked segregation of the markers, (ii) an ARS on a plasmid largely maintains sister chromatid attachment in meiosis I, and (iii) integration of these sequences at the LEU2 locus leads to chromosome breakage. Deletions performed on ARS18 show that CEN and ARS functions can be physically separated, but both are needed to establish a replicating plasmid.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barth G., Weber H. Improvement of sporulation in the yeast Yarrowia lipolytica. Antonie Van Leeuwenhoek. 1985;51(2):167–177. doi: 10.1007/BF02310010. [DOI] [PubMed] [Google Scholar]
  2. Bouton A. H., Smith M. M. Fine-structure analysis of the DNA sequence requirements for autonomous replication of Saccharomyces cerevisiae plasmids. Mol Cell Biol. 1986 Jul;6(7):2354–2363. doi: 10.1128/mcb.6.7.2354. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chen E. Y., Seeburg P. H. Supercoil sequencing: a fast and simple method for sequencing plasmid DNA. DNA. 1985 Apr;4(2):165–170. doi: 10.1089/dna.1985.4.165. [DOI] [PubMed] [Google Scholar]
  4. Clarke L., Carbon J. Isolation of a yeast centromere and construction of functional small circular chromosomes. Nature. 1980 Oct 9;287(5782):504–509. doi: 10.1038/287504a0. [DOI] [PubMed] [Google Scholar]
  5. Clarke L., Carbon J. The structure and function of yeast centromeres. Annu Rev Genet. 1985;19:29–55. doi: 10.1146/annurev.ge.19.120185.000333. [DOI] [PubMed] [Google Scholar]
  6. Clarke L. Centromeres of budding and fission yeasts. Trends Genet. 1990 May;6(5):150–154. doi: 10.1016/0168-9525(90)90149-z. [DOI] [PubMed] [Google Scholar]
  7. Cottarel G., Shero J. H., Hieter P., Hegemann J. H. A 125-base-pair CEN6 DNA fragment is sufficient for complete meiotic and mitotic centromere functions in Saccharomyces cerevisiae. Mol Cell Biol. 1989 Aug;9(8):3342–3349. doi: 10.1128/mcb.9.8.3342. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Dubey D. D., Davis L. R., Greenfeder S. A., Ong L. Y., Zhu J. G., Broach J. R., Newlon C. S., Huberman J. A. Evidence suggesting that the ARS elements associated with silencers of the yeast mating-type locus HML do not function as chromosomal DNA replication origins. Mol Cell Biol. 1991 Oct;11(10):5346–5355. doi: 10.1128/mcb.11.10.5346. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Fishel B., Amstutz H., Baum M., Carbon J., Clarke L. Structural organization and functional analysis of centromeric DNA in the fission yeast Schizosaccharomyces pombe. Mol Cell Biol. 1988 Feb;8(2):754–763. doi: 10.1128/mcb.8.2.754. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Fournier P., Guyaneux L., Chasles M., Gaillardin C. Scarcity of ars sequences isolated in a morphogenesis mutant of the yeast Yarrowia lipolytica. Yeast. 1991 Jan;7(1):25–36. doi: 10.1002/yea.320070104. [DOI] [PubMed] [Google Scholar]
  11. Gaillardin C. M., Charoy V., Heslot H. A study of copulation, sporulation and meiotic segregation in Candida lipolytica. Arch Mikrobiol. 1973;92(1):69–83. doi: 10.1007/BF00409513. [DOI] [PubMed] [Google Scholar]
  12. Haber J. E., Thorburn P. C., Rogers D. Meiotic and mitotic behavior of dicentric chromosomes in Saccharomyces cerevisiae. Genetics. 1984 Feb;106(2):185–205. doi: 10.1093/genetics/106.2.185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Heus J. J., Zonneveld B. J., de Steensma H. Y., van den Berg J. A. The consensus sequence of Kluyveromyces lactis centromeres shows homology to functional centromeric DNA from Saccharomyces cerevisiae. Mol Gen Genet. 1993 Jan;236(2-3):355–362. doi: 10.1007/BF00277133. [DOI] [PubMed] [Google Scholar]
  14. Heyer W. D., Sipiczki M., Kohli J. Replicating plasmids in Schizosaccharomyces pombe: improvement of symmetric segregation by a new genetic element. Mol Cell Biol. 1986 Jan;6(1):80–89. doi: 10.1128/mcb.6.1.80. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Hieter P., Mann C., Snyder M., Davis R. W. Mitotic stability of yeast chromosomes: a colony color assay that measures nondisjunction and chromosome loss. Cell. 1985 Feb;40(2):381–392. doi: 10.1016/0092-8674(85)90152-7. [DOI] [PubMed] [Google Scholar]
  16. Hill A., Bloom K. Acquisition and processing of a conditional dicentric chromosome in Saccharomyces cerevisiae. Mol Cell Biol. 1989 Mar;9(3):1368–1370. doi: 10.1128/mcb.9.3.1368. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Jäger D., Philippsen P. Stabilization of dicentric chromosomes in Saccharomyces cerevisiae by telomere addition to broken ends or by centromere deletion. EMBO J. 1989 Jan;8(1):247–254. doi: 10.1002/j.1460-2075.1989.tb03370.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Jørgensen P., Collins J., Fiil N., von Meyenbourg K. A ribosomal RNA gene, rrnC, of Escherichia coli, mapped by specialized transducing lambdadilv and lambda drbs phages. Mol Gen Genet. 1978 Jul 11;163(2):223–228. doi: 10.1007/BF00267413. [DOI] [PubMed] [Google Scholar]
  19. Kearsey S. Analysis of sequences conferring autonomous replication in baker's yeast. EMBO J. 1983;2(9):1571–1575. doi: 10.1002/j.1460-2075.1983.tb01626.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Kikuchi Y. Yeast plasmid requires a cis-acting locus and two plasmid proteins for its stable maintenance. Cell. 1983 Dec;35(2 Pt 1):487–493. doi: 10.1016/0092-8674(83)90182-4. [DOI] [PubMed] [Google Scholar]
  21. Kingsman A. J., Clarke L., Mortimer R. K., Carbon J. Replication in Saccharomyces cerevisiae of plasmid pBR313 carrying DNA from the yeast trpl region. Gene. 1979 Oct;7(2):141–152. doi: 10.1016/0378-1119(79)90029-5. [DOI] [PubMed] [Google Scholar]
  22. Lang-Hinrichs C., Dössereck C., Fath I., Stahl U. Use of the Tn903 neomycin-resistance gene for promoter analysis in the fission yeast Schizosaccharomyces pombe. Curr Genet. 1990 Dec;18(6):511–516. doi: 10.1007/BF00327021. [DOI] [PubMed] [Google Scholar]
  23. Meilhoc E., Masson J. M., Teissié J. High efficiency transformation of intact yeast cells by electric field pulses. Biotechnology (N Y) 1990 Mar;8(3):223–227. doi: 10.1038/nbt0390-223. [DOI] [PubMed] [Google Scholar]
  24. Murakami S., Matsumoto T., Niwa O., Yanagida M. Structure of the fission yeast centromere cen3: direct analysis of the reiterated inverted region. Chromosoma. 1991 Dec;101(4):214–221. doi: 10.1007/BF00365153. [DOI] [PubMed] [Google Scholar]
  25. Murphy M., Fitzgerald-Hayes M. Cis- and trans-acting factors involved in centromere function in Saccharomyces cerevisiae. Mol Microbiol. 1990 Mar;4(3):329–336. doi: 10.1111/j.1365-2958.1990.tb00600.x. [DOI] [PubMed] [Google Scholar]
  26. Newlon C. S. Yeast chromosome replication and segregation. Microbiol Rev. 1988 Dec;52(4):568–601. doi: 10.1128/mr.52.4.568-601.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Palzkill T. G., Newlon C. S. A yeast replication origin consists of multiple copies of a small conserved sequence. Cell. 1988 May 6;53(3):441–450. doi: 10.1016/0092-8674(88)90164-x. [DOI] [PubMed] [Google Scholar]
  28. Panzeri L., Landonio L., Stotz A., Philippsen P. Role of conserved sequence elements in yeast centromere DNA. EMBO J. 1985 Jul;4(7):1867–1874. doi: 10.1002/j.1460-2075.1985.tb03862.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Suzuki K., Imai Y., Yamashita I., Fukui S. In vivo ligation of linear DNA molecules to circular forms in the yeast Saccharomyces cerevisiae. J Bacteriol. 1983 Aug;155(2):747–754. doi: 10.1128/jb.155.2.747-754.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Zweifel S. G., Fangman W. L. Creation of ARS activity in yeast through iteration of non-functional sequences. Yeast. 1990 May-Jun;6(3):179–186. doi: 10.1002/yea.320060302. [DOI] [PubMed] [Google Scholar]

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