Table 1.
Name | Primer | Sequence (5′→3′) | Usage |
---|---|---|---|
poIRF1 | PB-IRF1-F | ACTGAATTCATGCCCATCACTCGGATGCGCAT | PiggyBac donor vector construction |
PB-IRF1-R | TACCTCGAGGGCCTACGGTGCACAAGGAAT | ||
IRF1-Q-F | GCACCAGCGACCTGTACAACT | RT-qPCR | |
IRF1-Q-R | TCCTCATCTGTTGCAGCTTCA | ||
GAPDH | GAPDH-Q-F | TGCCAACGTGTCGGTTGT | RT-qPCR |
GAPDH-Q-R | TGTCATCATATTTGGCAGGTTT | ||
siRNA | si-IRF1 | GGGCUGAUCUGGAUUAAUAdTdT | Knockdown |
si-Ctrl | UUCUCCGAACGUGUCACGUdTdT |