Figure 1.

Monocyte-derived macrophages. Monocytes were maintained in serum-free culture medium containing M-CSF or GM-CSF for 7 days. Then cells were directly observed in the culture plate under an inverted microscope (initial magnification X400) (a). Cells were cytocentrifuged on slides and stained with MGG (b) or labeled with anti-CD68 (c) and anti-CD163 (d) antibodies by immunocytochemistry (initial magnification X1000). Parental monocytes and derived macrophages were collected and analyzed by flow cytometre (e). These observations are representative of three independent experiments.