Skip to main content
Scientific Reports logoLink to Scientific Reports
. 2015 Dec 4;5:17671. doi: 10.1038/srep17671

Content and activity of human liver microsomal protein and prediction of individual hepatic clearance in vivo

Haifeng Zhang 1,*, Na Gao 1,*, Xin Tian 1, Tingting Liu 1, Yan Fang 1, Jun Zhou 1, Qiang Wen 1, Binbin Xu 1, Bing Qi 1, Jie Gao 1, Hongmeng Li 1, Linjing Jia 1, Hailing Qiao 1,a
PMCID: PMC4669488  PMID: 26635233

Abstract

The lack of information concerning individual variation in content and activity of human liver microsomal protein is one of the most important obstacles for designing personalized medicines. We demonstrated that the mean value of microsomal protein per gram of liver (MPPGL) was 39.46 mg/g in 128 human livers and up to 19-fold individual variations existed. Meanwhile, the metabolic activities of 10 cytochrome P450 (CYPs) were detected in microsomes and liver tissues, respectively, which showed huge individual variations (200-fold). Compared with microsomes, the activities of liver tissues were much suitable to express the individual variations of CYP activities. Furthermore, individual variations in the in vivo clearance of tolbutamide were successfully predicted with the individual parameter values. In conclusion, we offer the values for MPPGL contents in normal liver tissues and build a new method to assess the in vitro CYP activities. In addition, large individual variations exist in predicted hepatic clearance of tolbutamide. These findings provide important physiological parameters for physiologically-based pharmacokinetics models and thus, establish a solid foundation for future development of personalized medicines.


The liver is the main site of metabolic clearance in humans and is most often the focus of drug optimization and safety studies1. The cytochrome P450 (CYP) superfamily consists of 57 genes and constitutes the major enzyme system responsible for the metabolism of a diverse array of endogenous and exogenous compounds2,3. As a kind of membrane bound enzymes in eukaryotic cells, most of the CYPs, particularly those involved in the metabolism of drugs and xenobiotics, are located on the cytoplasmic side of the endoplasmic reticulum. With the development of tissue homogenate techniques and differential centrifugation methods, microsomal vesicles derived from the endoplasmic reticulum could be separated and subsequent evaluation of CYP enzymes showed their localization to microsomes4,5,6. Because of their reproducible nature, capacity for long-term storage, and extensive characterization of optimal incubation conditions, human liver microsomes (HLM) have become the dominant system used to characterize drug metabolism in vitro.

The amount of microsomal protein per gram of liver (MPPGL) is a critical scaling factor used in physiologically-based pharmacokinetics (PBPK) models to extrapolate in vitro rates of metabolism to drug clearance in vivo7,8,9. In order to assure the accuracy of predicted values for in vivo clearance, the MPPGL level should be determined precisely and individual variations in MPPGL should be considered. Unfortunately, to date there are only a limited number of studies concerning MPPGL amounts in human samples. Early estimates of MPPGL were limited because they were generated either from unmatched homogenate and microsomal samples10, or mean values of CYP content or cytochrome P450 oxidoreductase (POR) activity in homogenates and microsomes11. Several studies also used only a small number of livers12,13,14,15,16 and the background information for the samples was often incomplete. Moreover, the values for MPPGL reported by different groups used different tissue sources, different correction methods to account for losses of microsomal protein and relatively small sample sizes that in turn provided varying mean values16. These studies also did not give significant attention to the potential effects of individual variations in the MPPGL. Therefore, determination of the contents and individual variations in MPPGL over a large number of samples is needed in order to provide reliable physiological parameters for in vivo and in vitro research.

Traditionally, the in vitro metabolic activity of CYP is determined on the basis of per mg of microsomal protein (VM), not on per gram of liver (VL). However, the ultimate application of the in vitro metabolic study is within human tissue, the ability to obtain close estimates of in vivo behavior from the in vitro data is an important opportunity to be fully exploited. Though MPPGL were determined in relatively small size of liver tissues, considerable individual variations were found in MPPGL contents15,17. Compared with CYP activity based on liver tissue, VM has obvious disadvantage for the individual variation of activity might be underestimated, because the ignored MPPGL has larger individual variations. Consequently, the VL could be more appropriate to represent the in vitro metabolism of CYP and to assess the individual variation in CYP activities.

Given the potential for large individual variations in response to a given drug dose, substantial effort and expense may be expended during drug development, particularly for drugs that have narrow therapeutic windows, which can frequently cause severe toxicities and even death. If the safe therapeutic range of a given drug in a population can be predicted before initiating a clinical trial, the efficiency of drug development would be improved. The in vitro-in vivo extrapolation (IVIVE) method affords researchers the opportunity to produce quantitative data on drug metabolism prior to studying pharmacokinetics in vivo. Therefore, after the first demonstration of IVIVE in rats in 197718, many subsequent efforts were concentrated on this area. However, in these early studies there was a significantly predictive bias for in vivo clearance from the in vitro metabolic data because existing variation was not considered and instead mean parameter value reconstructed from very small data sets were used19,20,21,22,23. Hence, further efforts were made to incorporate population variability into PBPK models to predict in vivo clearance24,25,26.

Recently, the PBPK program in the Division of Pharmacometrics at the FDA decided to bring PBPK models into the drug review process (http://www.fda.gov/aboutfda/centersoffices /officeofmedicalproductsandtobacco/cder/ucm365118.htm). However, valid predictions for in vivo clearance that are based on PBPK models require large numbers of different individual parameters. Although many individual characteristics can influence the outcomes of these predictions, the greatest attention has been given to variations that occur in drug metabolism, particularly that mediated by the liver26. The five most important parameters in predicting hepatic clearances (CLH) are: i) MPPGL, ii) in vitro metabolic clearance (Inline graphic), iii) liver weight (LW), iv) hepatic blood flow (QH) and v) body weight (BW). Unfortunately, until now there have been no reports that used individual values for these important liver sample parameters to predict variations in in vivo clearance.

In order to assess the utility of individual parameters in predicting in vivo clearance rates, we assessed the metabolism of the sulfonylurea drug tolbutamide in liver samples. Tolbutamide is the probe of CYP2C9, which is one of the most abundant CYPs in human liver and is responsible for the metabolism of many drugs3. While the effects of genetic polymorphisms on CYP2C9 activities have been widely reported, experimental information demonstrating individual variations in tolbutamide metabolism in vitro are rather limited. An analysis of tolbutamide metabolism in HLM can be used not only to assess individual variations, but also to predict in vivo clearance rates. Such data will be informative for the design of personalized medicines.

In this study, 128 liver samples were collected to characterize individual variations in the contents of MPPGL and the metabolic activities of 10 CYPs based on microsomes (VM) and liver tissues (VL). For the first time, the distribution of MPPGL was assessed. The differences between VM and VL were compared and the correlations among VM or VL were analyzed. Furthermore, individual variations in the in vivo clearance of tolbutamide, used as a CYP2C9 probe substrate, were predicted using five important individual parameter values in large number of liver samples.

Results

Microsomal protein

Individual variation in contents

The values for MPPGL levels in 128 samples indicated a not-normal distribution, with minimal and maximal values of 6.71 and 127.95 mg microsomal protein per gram liver, representing a 19-fold variation (Fig. 1). The mean MPPGL content ± SD was about 39.46 ± 21.57 mg/g liver. The values of MPPGL at the 2.5th and the 97.5th percentiles were 10.5 and 102.82 mg/g liver, respectively, exhibiting about a 10-fold variation. There was one extreme value (127.95 mg/g liver) and four outliers (116.50, 104.23, 97.98 and 96.97 mg/g liver) in MPPGL content. Compared with the other samples analyzed, these samples showed no extraordinary characteristics, so it can be inferred that individuals with extreme values of MPPGL do indeed exist in the population.

Figure 1. Frequency distribution of microsomal protein per gram of liver in 128 human livers.

Figure 1

Effect of demographic factors and clinical data on contents

MPPGL content data were stratified by liver donor age, gender, smoking habit, alcohol consumption and tissue resource and then analyzed by Mann-Whitney U test or Kruskal-Wallis test. As shown in Table 1, MPPGL contents were not associated with either age (P > 0.05) or gender (P > 0.05). Smoking and alcohol consumption had no effects on MPPGL values (P > 0.05). We failed to detect marked disease-related differences in MPPGL contents (P > 0.05).

Table 1. Donor characteristics of human liver samples and MPPGL content in donor subgroups (n = 128).
Variables Group Number Percentage(%) MPPGLcontent (mg/g)(Mean ± SD)
Gender Male 40 31.3 41.50 ± 21.21
Female 88 68.8 38.53 ± 21.79
Age (years) 20–45 51 39.8 37.71 ± 21.31
46–60 63 49.2 40.59 ± 22.06
61–75 14 10.9 40.69 ± 21.45
Smoking Non-smoking 115 89.8 39.10 ± 21.86
Smoking 13 10.2 42.58 ± 19.30
Drinking Non-drinking 115 89.8 39.13 ± 21.93
Drinking 13 10.2 42.36 ± 18.64
Medical diagnosis Hepatic cavernous hemangioma 93 72.7 38.25 ± 21.93
Metastatic carcinoma 10 7.8 40.79 ± 19.07
Cholelithiasis 9 7.0 41.03 ± 28.73
Gallbladder cancer 5 3.9 50.91 ± 21.95
Hepatic cholangiocarcinoma 7 5.5 45.42 ± 15.45
Hepatocellular carcinoma 4 3.1 35.99 ± 12.68

The individual variation in metabolic activities of the CYP enzymes

The metabolic activities based on microsomes

Metabolic rates of 10 kinds of CYPs were detected in 78 liver microsomes using probe drugs known to be specific for each enzyme. The data of CYP activities shown in Table 2 and Fig. 2 demonstrated huge individual variations. The two biggest individual variations in VM took place in the activity of CYP2C19 and CYP2A6, reaching to 232 and 109-fold, followed by that of CYP3A4/5 CYP2D6, CYP2C8, CYP2B6, CYP1A2 and CYP2E1, demonstrating the fold-change of 72, 45, 31, 24, 24, and 11, respectively (Fig. 2a). Compared with other CYP members, fold-change of CYP2C9 VM was much lower but still achieving 6-fold. As shown in Fig. 2b, the highest variation (57-fold) in VM at 95% PI existed in the activity of CYP2A6, whereas the remaining enzymes had the following rank order: CYP2C19> CYP2D6> CYP2C8> CYP3A4/5> CYP2B6> CYP1A2> CYP2E1> CYP2C9 (28, 21, 17, 17, 12, 8, 5, and 5-fold, respectively).

Table 2. Metabolic activities of the individual CYP enzymes in human livers (n = 78).
  Probe drug (Metabolite)   VM (pmol/min/mg protein) VL (nmol/min/g liver)
CYP1A2 phenacetin (paracetamol) Median 677.94 22.12
Range 97.37–2368.14 3.16–119.91
95% PI 169.20–1344.69 4.77–71.38
CYP2A6 coumarin (7-OH-coumarin) Median 267.92 8.19
Range 7.20–788.56 0.22–47.08
95% PI 12.19–689.73 0.35–42.83
CYP2B6 bupropion (4-OH-bupropion). Median 46.67 1.68
Range 11.75–290.89 0.28–17.63
95% PI 12.16–151.14 0.31–11.31
CYP2C8 paclitaxel (6-OH- Paclitaxel) Median 31.50 0.96
Range 2.74–87.23 0.12–7.71
95% PI 4.34–74.27 0.17–4.81
CYP2C9 tolbutamide.(4-OH-tolbutamide) Median 222.70 7.29
Range 70.00–461.07 1.51–37.61
95% PI 76.17–391.49 1.96–34.92
CYP2C19 omeprazole (4-OH-omeprazole) Median 97.62 3.64
Range 1.40–325.03 0.14–19.58
95% PI 9.98–274.83 0.51–17.62
CYP2D6 dextromethorphan (3-methoxymorphinan) Median 68.16 2.11
Range 4.93–222.34 0.19–14.49
95% PI 9.06–190.03 0.30–14.15
CYP2E1 chlorzoxazone (6-OH-chlorzoxazone) Median 486.92 16.03
Range 140.81–1604.44 3.28–74.52
95% PI 198.24–1061.97 4.60–69.28
CYP3A4/5 midazolam (1-OH-midazolam) Median 836.56 29.05
Range 57.24–4144.93 2.93–251.26
95% PI 178.15–2967.83 3.55–217.79

VM: metabolic rate of the individual CYP enzyme based on per mg microsomal protein; VL: metabolic rate of the individual CYP enzyme based on per gram liver tissue; PI: percentile interval.

Figure 2. Fold-change of CYP metabolic activity based on microsomes (VM) or liver tissues (VL).

Figure 2

The individual fold-change is expressed as the ratio between the maximal and minimal values of CYP metabolic rate (a) or between the 97.5th and the 2.5th percentiles of the observed CYP metabolic rate (b).

The metabolic activities based on liver tissues

According to the contents of MPPGL determined above, individual metabolic rates per gram liver (VM) were obtained by multiplying the individual MPPGL values, representing the CYP activity of liver tissue (Table 2). However, when calculation of CYP activity based on liver tissue, the individual variations of CYP activities were even more pronounced (Fig. 2a). VL of CYP2A6 exhibited the largest individual variation (210-fold), followed by that of CYP2C19, showing 144-fold individual variation. The remaining enzymes had the following rank order: CYP3A4/5>CYP2D6>CYP2B6>CYP2C8> CYP1A2>CYP2C9>CYP2E1 (86, 77, 64, 63, 38, 25 and 23-fold, respectively). The biggest individual variation in VL at 95%PI took place in the activity of CYP2A6, reaching to 122, followed by CYP3A4/5, CYP2D6, CYP2B6, CYP2C19, CYP2C8, CYP2C9, CYP2E1, and CYP1A2, demonstrating the fold-change of 61, 46, 36, 34, 28, 18, 15 and 15, respectively (Fig. 2b).

The inter-individual variations in VM and VL

As shown in Fig.2a, inter-individual variations in the VL of CYPs were much higher than those of corresponding VM except CYP2C19. The fold-changes in VL of CYP2C9, CYP2B6, CYP2E1, CYP2C8, CYP2A6, CYP2D6, CYP1A2 and CYP3A4/5 exceeded those of corresponding CYP VM by 278%, 159%, 99%, 96%, 92%, 71%, 56% and 18%, respectively, However, the fold-change in VL of 2C19 decreased by 38% compared with that of CYP2C19 VM. When the fold-change expressed as ratio between 95% PI of the observed CYP metabolic activities (Fig. 2b), the inter-individual variation degree of VL in all the CYP isoforms were higher than the corresponding VM. Among 10 CYPs, VL of CYP2C9, CYP2B6, CYP2E1, CYP2C8, and CYP2A6 had the large amplitude of variation whereas those for CYP3A4/5 and CYP2C19 were less compared with corresponding VM (Fig. 2b). Additionally, in our study the metabolic rates of CYP2C19 and CYP2A6 showed the highest fold-changes of the measured enzymes; whereas those for CYP2C9, CYP1A2 and CYP2E1 were relatively lower (Fig. 2).

The intra-individual variations between VM and VL

The inter-individual variations of CYP metabolic activities in VM and VL were shown in Table 2 and Fig. 2. However, the intra-individual variation between VL and the corresponding VM in each CYP isoform of 78 samples was unknown and this situation was described by rank change percentage (Fig. 3). Rank change of individual VL compared with corresponding VM in 10 CYPs was obvious. For CYP1A2, CYP2C8, CYP2C9 and CYP2E1, the place change exceeding 20% accounted for 47%, 41%, 40% and 41% of the total samples, respectively, whereas those for the remaining CYPs were about 30%. Owing to the extremely high or low MPPGL amounts in some individuals, rank changes of certain CYP isoforms in these cases varied drastically. As shown in Fig. 3, the rank change of CYP2E1 in one sample experienced the most dramatic change, up 54 places (change of 69%). Next were CYP1A2 (2 cases), CYP2B6 (1 case), CYP2C9 (6 cases), CYP3A4 (1 case), CYP2C8 (2 cases), CYP2C19 (1 case), altering 41–51 places (change of 51–65%).

Figure 3. Percentage of rank change of individual VL compared with corresponding VM in 10 CYPs of 78 liver samples.

Figure 3

78 samples were ranked in ascending order according to the value of VM or VL for each CYP isoform, respectively. The rank change for each CYP isoform of each individual was the absolute difference value between the rank of VM and the rank of corresponding VL. The percentage of rank change was calculated by the total samples of 78 divided by rank change absolute value of each individual and every change of 10% as a group (such as 0%-10%, 10%-20%). The percentage of the samples in each group to total samples was also calculated. Rank change of less than 10% was considered as tiny change, between 10% and 20% as moderate change, between 20% and 50% as obvious change and exceeding 50% as dramatic change.

Effects of demographic factors and clinical data on CYP activities

Univariate analysis was performed to investigate whether demographic factors were associated with VM and VL (Fig. 4). There were no statistically significant differences (P > 0.05) in the metabolic activities of ten CYPs as a function of gender, age, smoking status, drinking habit or tissue resources with three exceptions. Statistically significant differences between VM of CYP1A2 in male and female donors (P = 0.003, n = 78) were seen, which was consistent with other studies27,28. Comparing with age 3 group (61–75 years old), the VM of CYP2C8 and CYP2C9 were significantly higher in age 1 (20–45 years old) and age 2 (46–60 years old) groups (Fig. 4b, c). Yang et al.29 also found age had a substantial impact on the activity of CYP2C9.

Figure 4. Influences of demographic factors on CYP activities.

Figure 4

Effects of sex on the metabolic activity of CYP1A2 (a), effects of age on the metabolic activity of CYP2C8 (b) and CYP2C9 (c) in liver microsomes. Data are shown as box plots representing medians with minimal and maximal values.

Association between MPPGL contents and CYP activities

Association based on microsomes

Spearman correlation analysis was used to identify the correlations between MPPGL contents and VM for 10 CYP, respectively, and the results showed there was no significant correlation between MPPGL contents and VM of 10 CYPs (P > 0.05) (Supplementary Fig. S1).

Association based on liver tissues

In contrast to VM, there were strong correlations (r≥0.6, P<0.001) between MPPGL contents and VL of CYPs besides CYP2A6, CYP3A4/5, CYP2C8 (moderate correlations, 0.3≤r<0.6, P<0.001) (Supplementary Fig. S2).

Prediction of tolbutamide hepatic clearance

In vitro clearance of tolbutamide

The mean ± SD of the Vmax and Km for tolbutamide 4-hydroxylation was 255.82 ± 79.48 (range 83.76 to 454.80) pmol/mg/min and 235.73 ± 99.78 (range 101.20 to 531.90) μM, respectively. Both the Vmax and Km values displayed individual variations of 5-fold, with the variations at 95% percentiles interval (PI) still reaching to 5-fold. The values of CLint, in vitro for 4-OH-tolbutamide by CYP2C9 are shown in Supplementary Table S1 and the not-normal distribution of the values displayed a 21-fold individual variation between the highest and lowest values.

All parameters used to predict the hepatic clearance of tolbutamide in the 78 HLM samples are listed in Supplementary Table S1. The variable degrees of the other three parameters were relatively lower, with only BW variations reaching 2-fold.

Prediction of tolbutamide hepatic clearance

The predicted and observed CLH for tolbutamide are shown in Table 3. The mean values for the predicted CLH of tolbutamide obtained by seven methods were all 0.113 ml/min/kg, but the predicted ranges showed obvious variations. Method G gave merely the mean value, whereas method A predicted the largest individual variation (51-fold between the highest and lowest values). Even at the 95% PI, the predicted variation still reached 30-fold with method A. Using only individual MPPGL or CLint,in vitro values, method B and C also displayed large individual variations (about 13- and 20-fold, respectively). In contrast, the ranges of the predicted CLH for tolbutamide were relatively narrow as calculated with method D, E and F, with less than 2-fold variation between the minimal and maximal values. The effects of individual values for the five parameters on the predicted CLH for tolbutamide are presented in Supplementary Fig. S3. Together these results indicate that ignoring individual variations in parameter values would lead to a failure to identify individuals who fall at the extremes of the population.

Table 3. Values for hepatic clearance of tolbutamide 4-hydroxylation and their variations.
  n Range Fold change (Max/Min) Mean ± SD 95% PI Fold change (95% PI)
Predicted CLH (ml/min/kg)
Method A 78 0.011–0.559 50.8 0.113 ± 0.095 0.012–0.365 30.4
Method B 78 0.027–0.341 12.6 0.113 ± 0.065 0.038–0.311 8.2
Method C 78 0.018–0.353 19.6 0.113 ± 0.064 0.026–0.268 10.3
Method D 78 0.093–0.142 1.5 0.113 ± 0.011 0.095–0.139 1.5
Method E 78 0.1128–0.1130 1.0 0.113 ± 0.000 0.1128–0.1130 1.0
Method F 78 0.079–0.161 2.0 0.116 ± 0.019 0.082–0.155 1.9
Method G 78 0.113
Observed CLH (ml/min/kg)
Back et al.47 7     0.260 ± 0.100    
Back et al.47 6     0.226 ± 0.024    
Back et al.47 6     0.239 ± 0.050    
Miners et al.48 6     0.171a    
Wilner et al.49 6     0.147 ± 0.013    
Wing et al.50 7     0.159a    

Method A used the individual values for each parameter (MPPGL, CLint, in vitro, LW, QH and BW) for 78 livers. Method B used the individual MPPGL value and the mean values for the remaining four parameters. Similar to method B, method C, D, E and F used the individual CLint, in vitro, LW, QH or BW value, respectively, and the mean values for the remaining four parameters. Method G used the mean value of the five parameters for that particular liver.

aNo SD is available. PI: percentile interval.

Prediction accuracy

As shown in Table 4, the mean average fold-error (AFE) values calculated by all of the seven methods (A-G) were the same (0.58), but the variable degrees of AFE were quite different. Method G gave merely the mean value, whereas method A predicted the largest individual variation in AFE (50-fold between the highest and lowest values). Even at the 95% PI, the predicted variation still reached 30-fold with method A. Using only individual MPPGL or CLint,in vitro values, method B and C also displayed large individual variation in AFE (about 13- and 20-fold, respectively). In contrast, the ranges in AFE values yielded by methods D, E and F were relatively narrow, with less than 2-fold variation between the minimal and maximal values. The percentage of predictions that fell within 2-fold of the in vivo value were different between different methods. By not incorporating the individual variation of these five parameters, or using the less variable degree in individual QH and LW, methods G, E and D provided overall accuracy. However, owing to the large individual variations seen for MPPGL and CLint,in vitro values, only 42%, 46% and 52% of the samples were within the 2-fold error as determined by method A, B and C, respectively.

Table 4. Comparing the accuracy of predictions using different methods.
Method AFE
Mean ± SD Range 95% PI % within of 2-fold error
A 0.58 ± 0.48 0.057–2.86 0.062–1.86 42
B 0.58 ± 0.33 0.14–1.74 0.20–1.59 46
C 0.58 ± 0.33 0.09–1.80 0.13–1.37 54
D 0.58 ± 0.06 0.47–0.72 0.48–0.71 92
E 0.58 ± 0.00 0.577–0.578 0.5767–0.5776 100
F 0.59 ± 0.10 0.40–0.82 0.42–0.79 82
G 0.58 100

AFE (average fold-error) was used to assess the accuracy of predictions while a two-fold precision limit corresponds to 0.5–2 of AFE values. Method A used individual values for each parameter (MPPGL, CLint, in vitro, LW, QH and BW) for 78 livers. Method B considered the individual MPPGL value and the mean values for the remaining four parameters. Similar to method B, methods C, D, E and F used the individual CLint, in vitro, LW, QH or BW value, respectively, and the mean value for the remaining four parameters. Method G used mean values for the five parameters for that particular liver. PI: percentile interval.

Discussion

This is the first extensive study to investigate the distribution of microsomal protein contents in a large number of normal liver samples. The not-normally distributed MPPGL values in 128 samples varied from 6.71 to 127.95 mg/g liver and the range at 95% PI were 10.5 to 102.82 mg/g. Meanwhile, the metabolic activities of 10 CYPs were detected in microsomes and liver tissues, respectively, which showed huge individual variations and the variation for some CYPs could reach over 200-fold. Compared with microsomes, the activities of liver tissues were much suitable to express the individual variations of CYP activities. Furthermore, individual variations in in vivo clearance of tolbutamide were successfully predicted with the individual parameter values and we provided a valuable database of metabolic parameters for normal livers for use in PBPK modeling.

Previous studies demonstrated that the CYP content and POR activity used for correcting the loss of MPPGL in homogenates are all essentially microsomal in origin30, and the corrected values of MPPGL based on either CYP contents or POR activities are comparable and not significantly different15,16. The average MPPGL value of 39.46 mg/g based on the POR activity from 128 liver tissues was essentially the same as the value obtained for 38 liver samples (40 mg/g16) and is in good agreement with the result of Pelkonen et al. (36 mg/g11). Thus, there is general agreement between various laboratories using a variety of methodologies. While our meangeo value of MPPGL is greater than the meangeo value of 28 mg/g determined by Barter et al.17, it is noteworthy that this result came from unpublished data and thus detailed information for the donors was not available. In addition, two other studies reported higher average MPPGL levels, in which the value of 77 mg/g was obtained from only four liver tissue samples12 and the determination of 53 mg/g was achieved by ELISA14. The use of ELISA to correct for microsomal losses is not a common approach, and this disparity may be due to differences in the correction methods used in these studies (i.e., POR activity vs. ELISA). In a previous study by this group a mean MPPGL value of 20 mg/g13 was obtained by using glucose-6-phosphatase activity to correct for microsomal loss.

To date only limited laboratory measurements concerning the variations in the contents of MPPGL were available and the largest reported variation was approximately 6-fold17. However, in the present study up to a 19-fold variation was detected and even at the 95% PI there was still a 10-fold variation, which is much higher than that reported in the literature. It should be emphasized that the present study used a larger number of samples and the range of MPPGL values obtained here overlapped with the results reported by other investigators16,17. The determination of MPPGL is time-consuming and requires the coordination of several operators. To ensure the accuracy of the MPPGL results, the effect of inter-operator differences on MPPGL values was investigated. Pooled human livers (n = 5) were respectively assessed by three operators in triplicate and the results showed that the difference in the MPPGL values obtained by different operators was not substantial and was less than 20%, which is in good agreement with a study by Wilson et al.31. As such, the large variation in the MPPGL levels seen in our study most likely represents the true biological variability present in the population. Taken together, our study offers physiological values for MPPGL in normal liver samples that have clear background information.

In general, the in vitro activity of CYP is based on microsomes (VM) and a number of studies have reported the high degrees of individual variations in the VM (such as 30-, 45-, 405-, 1790-, 30- and 124-fold for CYP2A6, CYP2B6, CYP2C19, CYP2D6, CYP2E1 and CYP3A428,32,33,34,35, respectively). Our study also found large individual variations in VM (Table 2 and Fig. 2). However, as the actual contents of MPPGL are unusually unknown, so far no reports are available regarding the metabolic activities of CYP on the basis of per gram liver (VL). Since the determination of MPPGL amount in our study, it has been found that the individual variations of CYP VL were much higher than those of corresponding VM. As shown in Fig. 2, the degree of the overall variation of VL for CYP2C9, CYP2B6, CYP2E1, CYP2C8 and CYP2A6 increased substantially comparing with those of corresponding VM of CYPs. Meanwhile, the intra-individual variation between VL and the corresponding VM in each CYP isoform of 78 samples was large. Especially for CYP1A2, CYP2C8, CYP2C9 and CYP2E1, more than 40% of the samples changed more than 20% of the total rank and for some cases, the rank change over more than 60% (Fig. 3). The reason for large rank change is due to the variation in MPPGL, especially the effect of extreme values in MPPGL. For example, the highest MPPGL contents in some cases resulted in the huge increase of rank whereas the lowest led to the dramatic decrease of rank. The change of the rank for each individual between VM and the corresponding VL indicated that VM didn’t equal to VL especially for the individuals with drastic rank changes. Taken together, it can be concluded that VL is preferred over the VM in representing the individual variations of CYP metabolic activity for the latter may mask the real individual variations of enzyme activities.

There are four stages in the IVIVE strategy, in which MPPGL, CLint, in vitro, LW, QH and BW are necessary parameters9. These parameters thus play a vital role in the accurate prediction of in vivo clearance rates. However, to our knowledge the various values for these important parameters used in the IVIVE were only single mean values7,36 and no consideration was given to variations in these parameters. Here, for the first time the individual values for the five of parameters were used to predict individual variations in hepatic tolbutamide clearance in a large number of liver samples. As shown in Table 3 and Supplementary Fig. S3, seven methods were designed to predict the hepatic clearance of tolbutamide. Because the mean and individual values of the five parameters used with the seven methods came from the same population, the mean values of the tolbutamide CLH predicted by each of the methods were the same. However, the variable degrees of the predicted CLH were quite different. Method G, which is a rather traditional “point” to “point” predictive manner, only provided the mean value and thus could not offer information on variations in the population. Method A, as a new approach that was used in this study, incorporated the individual variations of the five parameters into the prediction and in turn displayed as much as a 51-fold individual variation in the distribution of the predicted CLH for tolbutamide. Thus, the ability to predict individual variations in CLH is superior to the estimate of a mere mean. For future drug research and development, the prediction of the CLH range in the human body prior to the initiation of clinical studies can provide information about the effective range and toxic doses that can improve the efficiency of research on novel therapeutics. For older drugs that are currently in clinical use and have large individual variations, method A can help to re-assess the safety of these drugs and to provide a firm basis for the design of personalized treatments.

In contrast, methods B-F evaluated the effect of individual variation of a single parameter (MPPGL, CLint, in vitro, LW, QH or BW) on the prediction. Each parameter could affect the individual variation of the predicted CLH, but the contributions of the five parameters were different. The large individual variation predicted by method A was mainly caused by the variation in MPPGL and CLint,in vitro, so MPPGL and CLint,in vitro thus could play essential roles in the process of IVIVE. Among all the parameters used in IVIVE, individual values for MPPGL and CLint,in vitro were not easily obtained, therefore prediction of the individual variation in CLH would be highly difficult. In this study, individual values for the five kinds of important parameters were determined and used to predict the CLH of tolbutamide.

In order to assess the accuracy of the predictions, a comparison of the predicted hepatic clearance with the in vivo clearance in humans is needed. Due to a lack of human intravenous pharmacokinetic data in the Chinese population, data suitable for assessment of tolbutamide CLH predictions in Caucasian populations were selected from four previous studies (Table 3). As was previously known, CYP2C9 is highly polymorphic and the frequencies of CYP2C9*2 and CYP2C9*3, which exhibit poor enzymatic activity compared to CYP2C9*137, were higher in Caucasians compared to that in Chinese populations. Therefore, a reasonable assumption could be made that the metabolic activity of CYP2C9 should differ between Caucasian and Chinese populations. However, the CLint, in vitro of tolbutamide in Caucasian population (mean ± SD, range: 1.0 ± 0.2, 0.5–2.5; 1.35 ± 1.23, 0.27–4.0 μl/mg/min) were reported by McGinnity et al.38 and Carlile et al.39, respectively, which were in good agreement with those of Chinese people (mean ± SD, range: 1.32 ± 0.75, 0.20–4.18 μl/mg/min, this study). The consistency of the Inline graphic of tolbutamide in these two ethnic groups might suggest that gene polymorphisms in CYP2C9 have little effect on the metabolism of tolbutamide in ethnic Chinese and Caucasians. Consequently, the in vivo CLH for tolbutamide derived from the Western population should be suitable for comparisons with the predicted CLH for tolbutamide in Chinese patients. The predicted mean value within 2-fold of actual values showed that each of the methods was accurate in predicting the CLH of tolbutamide (Table 4).

However, more than half of the samples fell outside of the 2-fold error for method A, B and C, which may explain, at least in part, the bias that exists in most IVIVE studies. Traditionally, IVIVE employed only mean values to make predictions and as such cannot provide a range of AFE. In fact, as observed in our study, large variations indeed exist in many drug metabolism steps, so a method that incorporates into the IVIVE individual variations in each step can be a suitable way to make accurate predictions for in vivo CLH.

In conclusion, MPPGL values were determined and considerable individual variations in the contents were found in Chinese population. The metabolic activity of CYP based on liver tissue is a new method to assess the in vitro metabolic activity of CYP and superior to the metabolic activity of CYP based on microsomes. For the first time the individual values of five different parameters were used to predict individual variations in hepatic clearance in a large number of liver samples and variations in the in vivo clearance rates of tolbutamide were successfully predicted. These findings provide important physiological parameters for PBPK and furthermore, build a solid foundation for future development of personalized medicines.

Methods

Materials and Chemicals

All probe drugs and part metabolites used in this work were purchased from the National Institute for the Food and Drug Control (China), including phenacetin, coumarin, bupropion, paclitaxel, tolbutamide, omeprazole, dextromethorphan, chlorzoxazone, midazolam and paracetamol (phenacetin metabolite). Other metabolites (7-OH-coumarin, 4-OH-bupropion, 6-OH-Paclitaxel, 4-OH-tolbutamide, 4-OH-omeprazole, 3-methoxymorphinan, 6-OH-chlorzoxazone and 1-OH-midazolam) were obtained from Toronto Research Chemicals, Inc. (Canada). Methanol and acetonitrile were HPLC grade and were purchased from Siyou Chemical Reagent Co. (China). Reduced nicotinamide adenine dinucleotide phosphate (NADPH) and horse cytochrome C were obtained from Solarbio Science and Technology co. (China).

Human liver samples

One hundred and twenty-three Chinese liver tissues were previously collected40 from patients undergoing liver surgery during 2012 and 2014 in the first affiliated hospital of Zhengzhou University, the People’s Hospital of Henan Province, and the Tumors’ Hospital of Henan Province, respectively, besides 5 newly collected samples. The study was approved by the ethics committees of Zhengzhou University and written informed consent was obtained from each patient. All experiments were performed in accordance with the approved guidelines of Zhengzhou University ethics committees. Detailed information for each patient was well-documented and included gender, age, body height, body weight, smoking habits, alcohol consumption, clinical diagnosis, regular drug intake before surgery, previous history, allergic history, pathological diagnosis, imaging examination and laboratory test data (including, but not limited to, results from routine blood analysis, liver function tests and renal function tests). Liver samples from tumorous patients were 2 cm distant from the tumor tissues. Samples from normal livers were collected, with liver health confirmed by liver function tests, histopathological analysis and imaging examination by ultrasonography or CT. All liver samples were frozen immediately after removal and stored in liquid nitrogen until use.

Preparation of liver microsomes

Tissue samples were thawed on ice and weighed. The samples were finely homogenized on ice using a glass homogenizer in 0.05 M Tris-HCl (pH 7.0) buffer containing 1.12% w/v KCl and 1.12% v/v EDTA (10 ml per gram liver). After mixing, 0.5 ml of the homogenate was retained for POR activity analysis while the remaining sample was centrifuged at 9,000 × g for 20 min at 4 °C. The supernatant was collected and centrifuged at 100,000 × g for 1 hour at 4 °C with a Beckman Optima L-100K ultracentrifuge. The resulting microsomal pellet was resuspended in 0.15 M Tris-HCl (pH 7.6) buffer and centrifuged for an additional hour at 100,000 × g at 4 °C. The final microsome pellet was suspended in 0.25 M sucrose (2 ml per gram original sample). Both the homogenate and microsomal suspension were frozen in liquid nitrogen and stored at −80 °C until analysis. Microsomal protein concentrations were determined according to the Bradford method.

Determination of microsomal protein per gram of liver (MPPGL) levels

The activity of POR as measured in homogenates and microsomes produced from the same liver tissue sample was used to estimate the amount of MPPGL41. The POR assay is based on the rate of cytochrome C reduction by liver microsomes42. The reaction was conducted in 200 μl solution with 0.3 M potassium phosphate buffer (pH 7.7), 0.2 mM horse cytochrome C, and 5 μg microsomal proteins. Reactions were initiated by the addition of 20 μl 10 mM NADPH to 200 μl assay mixture for a total volume of 220 μl. The rate of cytochrome C reduction was determined from the rate of increase in absorbance at 550 nm produced by the reduced form of cytochrome C using a BioTek Synergy H1MD Multi-Mode microplate reader in the kinetic mode before and after the addition of NADPH (0–5 min). MPPGL values were calculated with the following equation15: MPPGL = {rate of reductionhomogenate (nM/min/g liver)}/{rate of reductionmicrosome (nM/min/mg microsomal protein)}.

Measurement of CYP metabolic activities

Marker activities selective for individual CYP isoforms were determined at single concentrations in individual assays by incubation of 0.2–0.5 mg microsomal protein, 1 mM NADPH and the respective substrate (400 μM phenacetin for CYP1A2, 20 μM coumarin for CYP2A6, 500 μM bupropion for CYP2B6, 40 μM paclitaxel for CYP2C8, 1500 μM tolbutamide for CYP2C9, 250 μM omeprazole for CYP2C19, 320 μM dextromethorphan for CYP2D6, 500 μM chlorzoxazone for CYP2E1 and 50 μM midazolam for CYP3A4/5). In addition, seven different concentrations of tolbutamide (31.25 to 2000 μM) were examined to determine the Vmax and Km of 4-OH-tolbutamide and the in vitro CLint of tolbutamide was calculated using the following equation: CLint in vitro  = Vmax/Km. Incubation conditions were ensured linear metabolite formation with respect to reaction time and protein concentration. Each reaction was terminated after specified incubation period by adding 20 μl ice-cold acetonitrile or 1 ml ethyl acetate or perchloric acid and metabolite concentrations were determined by HPLC-UV or HPLC-FLD.

Prediction of tolbutamide hepatic clearance

The CLint,in vitro values obtained for tolbutamide were scaled to in vivo clearance by the following equations. The whole liver intrinsic clearance (CLint, liver) was estimated as:

graphic file with name srep17671-m3.jpg

Where LW is liver weight and BW refers to body weight. According to the body weight given for each patient, the liver weight (LW) was calculated from the liver volume (LV) multiplied by liver density, where LV (ml) = 12.5 × BW (kg) + 536.443 and liver density is 1.001 g/ml44. The formula for liver volume was derived from data collected from a Chinese population.

The hepatic clearance (CLH) of tolbutamide hydroxylation was then predicted using the well-stirred model:

graphic file with name srep17671-m4.jpg

Where fub is fraction unbound in blood (0.0982)8,45 and QH is liver blood flow, which is often expressed as a percentage of cardiac output because of the difficulty in determining the QH. QH was assumed to be 24.5% of cardiac output7. The values for cardiac output originated from data for normal Han Chinese males (n = 783) and females (n = 805) and the mean values from each group were selected according to the age and gender of the donors in this study46.

Both the mean and individual values for the five different parameters observed in this study (MPPGL, CLint, in vitro, LW, QH and BW) were used to predict the hepatic clearance of tolbutamide. According to the different combinations of mean and individual values of the five parameters, seven methods were employed. Method A used individual values for each parameter for that particular liver (n = 78). Method B used the individual MPPGL value and the mean value of the remaining four parameters. Similar to method B, methods C, D, E and F used the individual CLint, in vitro, LW, QH or BW value, respectively, and the mean value of the remaining four parameters. Method G used the mean value of the five parameters for 78 livers.

Due to a scarcity of data for the Chinese population, observed intravenous clearance values for tolbutamide were obtained from in vivo studies performed with healthy Caucasian subjects.

The accuracy of the predictions was assessed from the average fold-error (AFE). A two-fold precision limit corresponds to 0.5–2 of AFE values, where AFE = 10(∑logPredicted/Observed)/N 21. N refers to the number of separate reports in the literature concerning tolbutamide intravenous clearance.

Statistical Analyses

The normality of the data distribution was assessed using the method of Kolmogorov-Smirnov and Shapiro-Wilk. Because most data sets were not normally distributed, nonparametric methods were generally used for statistical analyses. The Mann-Whitney U test was used for pairwise comparison and the Kruskal-Wallis H test was applied for multiple pairwise comparisons. Non-parametric Spearman rank correlation analysis was performed to calculate the correlation coefficient (r). A P value < 0.05 was considered statistically significant (two-tailed). SPSS statistics 17 software was used for data management and statistical analyses. Graphs were generated using GraphPad Prism software 5.04.

Additional Information

How to cite this article: Zhang, H. et al. Content and activity of human liver microsomal protein and prediction of individual hepatic clearance in vivo. Sci. Rep. 5, 17671; doi: 10.1038/srep17671 (2015).

Supplementary Material

Supplementary Information
srep17671-s1.pdf (980.6KB, pdf)

Acknowledgments

This work was partly supported by the National Natural Science Foundation of China (No. 81473279), Science and Technology Innovative Scholar Program of Henan Province (No. 134200510019) and Scientific and Technical Innovation Team of Zhengzhou City (131PCXTD604). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Footnotes

Author Contributions H.Q. designed the experiments. H.Z. and H.Q. wrote the manuscript. N.G., X.T., Y.F. and J.Z. performed the kinetic analysis. H.Z. and T.L. performed the in vitro-in vivo extrapolation. H.Z., Y.F., T.L., B.X., B.Q., J.G., H.L. and L.J. prepared the human liver microsomes. H.Z., N.G. and Q.W. performed the data analysis. J.Z., T.L., Y.F., B.X., B.Q., J.G. and H.L. collected the liver samples. All authors read and approved the final manuscript.

References

  1. Zhang H., Davis C. D., Sinz M. W. & Rodrigues A. D. Cytochrome P450 reaction-phenotyping: an industrial perspective. Expert Opin Drug Metab Toxicol. 3, 667–687 (2007). [DOI] [PubMed] [Google Scholar]
  2. Zanger U. M. & Schwab M. Cytochrome P450 enzymes in drug metabolism: regulation of gene expression, enzyme activities, and impact of genetic variation. Pharmacol Ther. 138, 103–141 (2013). [DOI] [PubMed] [Google Scholar]
  3. Martiny V. Y. & Miteva M. A. Advances in molecular modeling of human cytochrome P450 polymorphism. J Mol Biol. 425, 3978–3992 (2013). [DOI] [PubMed] [Google Scholar]
  4. Ekins S., Ring B. J., Grace J., McRobie-Belle D. J. & Wrighton S. A. Present and future in vitro approaches for drug metabolism. J Pharmacol Toxicol Methods. 44, 313–324 (2000). [DOI] [PubMed] [Google Scholar]
  5. Reed J. R. & Backes W. L. Formation of P450. P450 complexes and their effect on P450 function. Pharmacol Ther. 133, 299–310 (2012). [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fujiki Y., Hubbard A. L., Fowler S. & Lazarow P. B. Isolation of intracellular membranes by means of sodium carbonate treatment: application to endoplasmic reticulum. J Cell Biol. 93, 97–102 (1982). [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Barter Z. E., Tucker G. T. & Rowland-Yeo K. Differences in cytochrome p450-mediated pharmacokinetics between chinese and caucasian populations predicted by mechanistic physiologically based pharmacokinetic modelling. Clin Pharmacokinet. 52, 1085–1100 (2013). [DOI] [PubMed] [Google Scholar]
  8. Poulin P., Kenny J. R., Hop C. E. & Haddad S. In vitro-in vivo extrapolation of clearance: modeling hepatic metabolic clearance of highly bound drugs and comparative assessment with existing calculation methods. J Pharm Sci. 101, 838–851 (2012). [DOI] [PubMed] [Google Scholar]
  9. Houston J. B. Utility of in vitro drug metabolism data in predicting in vivo metabolic clearance. Biochem Pharmacol. 47, 1469–1479 (1994). [DOI] [PubMed] [Google Scholar]
  10. Schoene B., Fleischmann R. A., Remmer H. & von Oldershausen H. F. Determination of drug metabolizing enzymes in needle biopsies of human liver. Eur J Clin Pharmacol. 4, 65–73 (1972). [DOI] [PubMed] [Google Scholar]
  11. Pelkonen O., Kaltiala E. H., Larmi T. K. & Karki N. T. Comparison of activities of drug-metabolizing enzymes in human fetal and adult livers. Clin Pharmacol Ther. 14, 840–846 (1973). [DOI] [PubMed] [Google Scholar]
  12. Baarnhielm C., Dahlback H. & Skanberg I. In vivo pharmacokinetics of felodipine predicted from in vitro studies in rat, dog and man. Acta Pharmacol Toxicol (Copenh). 59, 113–122 (1986). [DOI] [PubMed] [Google Scholar]
  13. Lipscomb J. C. & Fisher J. W., Confer, P.D. & Byczkowski, J.Z. In vitro to in vivo extrapolation for trichloroethylene metabolism in humans. Toxicol Appl Pharmacol. 152, 376–387 (1998). [DOI] [PubMed] [Google Scholar]
  14. Lipscomb J. C., Teuschler L. K., Swartout J. C., Striley C. A. & Snawder J. E. Variance of Microsomal Protein and Cytochrome P450 2E1 and 3A Forms in Adult Human Liver. Toxicol Mech Methods. 13, 45–51 (2003). [DOI] [PubMed] [Google Scholar]
  15. Wilson Z. E. et al. Inter-individual variability in levels of human microsomal protein and hepatocellularity per gram of liver. Br J Clin Pharmacol. 56, 433–440 (2003). [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Hakooz N. et al. Determination of a human hepatic microsomal scaling factor for predicting in vivo drug clearance. Pharm Res. 23, 533–539 (2006). [DOI] [PubMed] [Google Scholar]
  17. Barter Z. E. et al. Scaling factors for the extrapolation of in vivo metabolic drug clearance from in vitro data: reaching a consensus on values of human microsomal protein and hepatocellularity per gram of liver. Curr Drug Metab. 8, 33–45 (2007). [DOI] [PubMed] [Google Scholar]
  18. Rane A., Wilkinson G. R. & Shand D. G. Prediction of hepatic extraction ratio from in vitro measurement of intrinsic clearance. J Pharmacol Exp Ther. 200, 420–424 (1977). [PubMed] [Google Scholar]
  19. Iwatsubo T. et al. Prediction of in vivo drug metabolism in the human liver from in vitro metabolism data. Pharmacol Ther. 73, 147–171 (1997). [DOI] [PubMed] [Google Scholar]
  20. Nestorov I., Gueorguieva I., Jones H. M., Houston B. & Rowland M. Incorporating measures of variability and uncertainty into the prediction of in vivo hepatic clearance from in vitro data. Drug Metab Dispos. 30, 276–282 (2002). [DOI] [PubMed] [Google Scholar]
  21. Ito K. & Houston J. B. Prediction of human drug clearance from in vitro and preclinical data using physiologically based and empirical approaches. Pharm Res. 22, 103–112 (2005). [DOI] [PubMed] [Google Scholar]
  22. Riley R. J., McGinnity D. F. & Austin R. P. A unified model for predicting human hepatic, metabolic clearance from in vitro intrinsic clearance data in hepatocytes and microsomes. Drug Metab Dispos. 33, 1304–1311 (2005). [DOI] [PubMed] [Google Scholar]
  23. Hallifax D., Foster J. A. & Houston J. B. Prediction of human metabolic clearance from in vitro systems: retrospective analysis and prospective view. Pharm Res. 27, 2150–2161 (2010). [DOI] [PubMed] [Google Scholar]
  24. Howgate E. M., Rowland Yeo K., Proctor N. J., Tucker G. T. & Rostami-Hodjegan A. Prediction of in vivo drug clearance from in vitro data. I: impact of inter-individual variability. Xenobiotica. 36, 473–497 (2006). [DOI] [PubMed] [Google Scholar]
  25. Inoue S. et al. Prediction of in vivo drug clearance from in vitro data. II: potential inter-ethnic differences. Xenobiotica. 36, 499–513 (2006). [DOI] [PubMed] [Google Scholar]
  26. Rostami-Hodjegan A. & Tucker G. T. Simulation and prediction of in vivo drug metabolism in human populations from in vitro data. Nat Rev Drug Discov. 6, 140–148 (2007). [DOI] [PubMed] [Google Scholar]
  27. Shimada T., Yamazaki H., Mimura M., Inui Y. & Guengerich F. P. Interindividual variations in human liver cytochrome P-450 enzymes involved in the oxidation of drugs, carcinogens and toxic chemicals: studies with liver microsomes of 30 Japanese and 30 Caucasians. J Pharmacol Exp Ther. 270, 414–423 (1994). [PubMed] [Google Scholar]
  28. Yang J. et al. Metabolic capabilities of cytochrome P450 enzymes in Chinese liver microsomes compared with those in Caucasian liver microsomes. Br J Clin Pharmacol. 73, 268–284 (2012). [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Yang X. et al. Systematic genetic and genomic analysis of cytochrome P450 enzyme activities in human liver. Genome Res. 20, 1020–1036 (2010). [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Carlile D. J., Zomorodi K. & Houston J. B. Scaling factors to relate drug metabolic clearance in hepatic microsomes, isolated hepatocytes, and the intact liver: studies with induced livers involving diazepam. Drug Metab Dispos. 25, 903–911 (1997). [PubMed] [Google Scholar]
  31. Barter Z. E. et al. Covariation of human microsomal protein per gram of liver with age: absence of influence of operator and sample storage may justify interlaboratory data pooling. Drug Metab Dispos. 36, 2405–2409 (2008). [DOI] [PubMed] [Google Scholar]
  32. He P., Court M. H., Greenblatt D. J. & von Moltke L. L. Factors influencing midazolam hydroxylation activity in human liver microsomes. Drug Metab Dispos. 34, 1198–1207 (2006). [DOI] [PubMed] [Google Scholar]
  33. Mohri T. et al. Human CYP2E1 is regulated by miR-378. Biochem Pharmacol. 79, 1045–1052 (2010). [DOI] [PubMed] [Google Scholar]
  34. Wang H. et al. Association analysis of CYP2A6 genotypes and haplotypes with 5-fluorouracil formation from tegafur in human liver microsomes. Pharmacogenomics. 12, 481–492 (2011). [DOI] [PubMed] [Google Scholar]
  35. Yoshida R. et al. Effects of polymorphism in promoter region of human CYP2A6 gene (CYP2A6*9) on expression level of messenger ribonucleic acid and enzymatic activity in vivo and in vitro. Clin Pharmacol Ther. 74, 69–76 (2003). [DOI] [PubMed] [Google Scholar]
  36. Gill K. L., Houston J. B. & Galetin A. Characterization of in vitro glucuronidation clearance of a range of drugs in human kidney microsomes: comparison with liver and intestinal glucuronidation and impact of albumin. Drug Metab Dispos. 40, 825–835 (2012). [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Dai D. P. et al. In vitro functional characterization of 37 CYP2C9 allelic isoforms found in Chinese Han population. Acta Pharmacol Sin. 34, 1449–1456 (2013). [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. McGinnity D. F., Parker A. J., Soars M. & Riley R. J. Automated definition of the enzymology of drug oxidation by the major human drug metabolizing cytochrome P450s. Drug Metab Dispos. 28, 1327–1334 (2000). [PubMed] [Google Scholar]
  39. Carlile D. J., Hakooz N., Bayliss M. K. & Houston J. B. Microsomal prediction of in vivo clearance of CYP2C9 substrates in humans. Br J Clin Pharmacol. 47, 625–635 (1999). [DOI] [PMC free article] [PubMed] [Google Scholar]
  40. Zhang H. et al. Effect of Cytochrome b5 Content on the Activity of Polymorphic CYP1A2, 2B6, and 2E1 in Human Liver Microsomes. PLoS One. 10, e0128547 (2015). [DOI] [PMC free article] [PubMed] [Google Scholar]
  41. De Bock L. et al. Microsomal protein per gram of liver (MPPGL) in paediatric biliary atresia patients. Biopharm Drug Dispos. 35, 308–312 (2014). [DOI] [PubMed] [Google Scholar]
  42. Guengerich F. P., Martin M. V., Sohl C. D. & Cheng Q. Measurement of cytochrome P450 and NADPH-cytochrome P450 reductase. Nat Protoc. 4, 1245–1251 (2009). [DOI] [PMC free article] [PubMed] [Google Scholar]
  43. Wang X. F. et al. [Establishment of formula predicting adult standard liver volume for liver transplantation]. Zhonghua Wai Ke Za Zhi. 46, 1129–1132 (2008). [PubMed] [Google Scholar]
  44. Yuan D. et al. Estimation of standard liver volume for liver transplantation in the Chinese population. Transplant Proc. 40, 3536–3540 (2008). [DOI] [PubMed] [Google Scholar]
  45. Tassaneeyakul W. et al. Co-regulation of phenytoin and tolbutamide metabolism in humans. Br J Clin Pharmacol. 34, 494–498 (1992). [PMC free article] [PubMed] [Google Scholar]
  46. Li G. F., Yu G., Liu H. X. & Zheng Q. S. Ethnic-specific in vitro-in vivo extrapolation and physiologically based pharmacokinetic approaches to predict cytochrome P450-mediated pharmacokinetics in the Chinese population: opportunities and challenges. Clin Pharmacokinet. 53, 197–202 (2014). [DOI] [PubMed] [Google Scholar]
  47. Back D. J., Tjia J., Monig H., Ohnhaus E. E. & Park B. K. Selective inhibition of drug oxidation after simultaneous administration of two probe drugs, antipyrine and tolbutamide. Eur J Clin Pharmacol. 34, 157–163 (1988). [DOI] [PubMed] [Google Scholar]
  48. Miners J. O., Wing L. M., Lillywhite K. J. & Smith K. J. Failure of ‘therapeutic’ doses of beta-adrenoceptor antagonists to alter the disposition of tolbutamide and lignocaine. Br J Clin Pharmacol. 18, 853–860 (1984). [DOI] [PMC free article] [PubMed] [Google Scholar]
  49. Wilner K. D. & Gardner M. J. Cimetidine does not alter the clearance or plasma binding of tenidap in healthy male volunteers. Br J Clin Pharmacol. 39 Suppl 1, 21S–24S (1995). [DOI] [PMC free article] [PubMed] [Google Scholar]
  50. Wing L. M. & Miners J. O. Cotrimoxazole as an inhibitor of oxidative drug metabolism: effects of trimethoprim and sulphamethoxazole separately and combined on tolbutamide disposition. Br J Clin Pharmacol. 20, 482–485 (1985). [DOI] [PMC free article] [PubMed] [Google Scholar]

Associated Data

This section collects any data citations, data availability statements, or supplementary materials included in this article.

Supplementary Materials

Supplementary Information
srep17671-s1.pdf (980.6KB, pdf)

Articles from Scientific Reports are provided here courtesy of Nature Publishing Group

RESOURCES