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. Author manuscript; available in PMC: 2016 Dec 4.
Published in final edited form as: Glia. 2015 Jun 4;63(11):1982–1996. doi: 10.1002/glia.22868

Figure 5. Immunohistochemical staining of chronic reactive microglia.

Figure 5

Microglia chronically express MHC Class II and PD-L1 following MCMV brain infection. (A) Infected Foxp3-DTR-GFP transgenic mice with (+DTx) and without (−DTx) DTx treatment were perfused and brains were cryosectioned for immunohistochemistry. Co-labeling of MHC II (green), as an indicator of cell reactivity, and the microglial cell marker Iba-1 (red) was observed in the meninges and brain parenchyma at 40 dpi. (B) Elevated MHC Class II protein levels in chronically-infected brains with or without Treg depletion (±DTx) were quantified using ELISA. Pooled data presented are derived from three to nine animals/group at 40 dpi. (C) Brain sections from infected animals ±DTx treatment stained for Iba-1 at 24 dpi displayed microglial nodules with reactive morphology (white arrow). **P ≤ 0.01 vs. uninfected. ††P ≤ 0.01 vs. MCMV.