Skip to main content
. 2015 Aug 27;6(26):21892–21905. doi: 10.18632/oncotarget.5260

Figure 3. hTERT-eGFP-high MCF7 cells form mammospheres more efficiently, in a telomerase-dependent manner.

Figure 3

MCF7-hTERT-eGFP cells were fractionated into GFP-high (top 5%) and GFP-low (negative) groups. Then, five thousands cells from each group were seeded per well in 6-well low-attachment plates. These two groups were also compared to the total unfractionated cell population. Note that GFP-high cells form mammospheres with an efficiency nearly 2.5-fold greater than GFP-negative cells and the total unfractionated cell population. In this context, mammosphere formation was sensitive to that administration of a well-characterized telomerase inhibitor, namely MST-312, as expected. MFE, mammosphere forming efficiency.