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. 2015 Jun 10;6(26):22934–22948. doi: 10.18632/oncotarget.4473

Figure 1. Treatment with ML327 reduces cancer cell invasive potential but has no effect on cell viability.

Figure 1

A. Chemical structures of ML327 and its inactive analogue, 266Y. Effective dose is listed for each compound. B. Representative in cell western (ICW) plate showing concentration-dependent changes in E-cadherin protein (green) relative to β-actin (red) following treatment with ML327 concentration as indicated. The graph shows mean values with standard error bars from 3 replicate plates. C. SW620inv and H520 cells were cultured in the presence of 10μM ML327, 10μM 266Y or DMSO for up to 4 days. Individual wells (n = 4 per group) were harvested for DNA content measures by fluorometry at 24 hour intervals. Mean fluorescence units (FU) is graphed with standard deviations for replicate wells in a representative experiment. The graphs are representative of at least three separate experiments with similar results. D. TOP: Images (200x magnification) of invading fluorescently labeled SW620inv and H520 cells cultured on Matrigel-coated transwells in the presence of 10μM ML327, 10μM 266Y or DMSO. BOTTOM: The proportion of stained cells that invaded through the transwell is graphed with a bar indicating the mean value and the whiskers indicating the standard deviation for 3 replicate wells in a representative experiment, statistical significance was calculated using unpaired t test, ** indicates p < 0.005, * indicates p < 0.05. The graphed data is representative of at least three separate experiments with similar results.