Figure 5. Administration of honokiol by oral gavage inhibits the growth of FaDu and SCC-1 cells grown as tumor xenograft in athymic nude mice.

A. Body weight of mice in different treatment groups were recorded on weekly basis. The body weight of mice in control (vehicle-treated) and honokiol (100 mg/kg body weight) treated groups did not vary significantly throughout the duration of in vivo tumor xenograft experiment. B. Size of the tumor xenografts were recorded on weekly basis in each treatment group. Average tumor volume (mm³) ± SD (n = 6) was calculated from each group and was plotted as a function of time. Significant inhibition in tumor xenograft growth was recorded in the honokiol treated mice compared with the mice treated with vehicle alone. Significant difference vs control group at the termination of the experiment, P < 0.01 or P < 0.001. C. At the termination of the experiment, total tumor xenograft tissue was harvested from each mouse and the wet tumor weight was measured on a digital balance. Tumor weight is reported as the mean ± SD (g), n = 6. Significant inhibition in tumor weight in honokiol treated mice vs vehicle-treated control mice, P < 0.001.