Figure 1.

Thermocouple implant, behavior arena, and methods used for setup standardization. a, Schematic of rat’s skull with thermocouple implant. b, Schematic of the behavior box with overhead camera. Compartments are indicated from C1 to C5. There are dummy compartments between C1 and the wall, and C5 and the wall, to keep the odor flow away from the walls. c, Cross section of the behavior box and placement of anemometer at grid lines for airflow measurement. d, Odor plume visualization using planar green laser light. e, Olfactometer design in baseline IAA tracking experiments. f, Olfactometer design with background odor experiments. g, Specified boundary regions for defining trial outcome and strategy, color coded for each compartment. h, Projected odor path from each compartment used for calculation for deviation of trajectory from odor trail, color coded from C1–C5.