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. Author manuscript; available in PMC: 2016 Dec 3.
Published in final edited form as: Cell. 2015 Dec 3;163(6):1468–1483. doi: 10.1016/j.cell.2015.11.020

Figure 5. Orb2 monomer reduces whereas Orb2 oligomer stabilizes polyA tail.

Figure 5

(A) Orb2 promotes deadenylation of wild type Teq3′UTR. Mutation of the M2 sequence reduces deadenylation. (B) Deadenylation is reduced in extracts lacking Orb2 (Δorb2 or Orb2ΔQ) compared to wild type extract. (C) Only Orb2 monomer but not (D) Orb2 oligomer efficiently deadenylates the 3′UTR. (E) Orb2 oligomer stabilizes and polyadenylates capped and short polyA tailed mRNA in vitro. (F) PCR based PolyA tail (PAT) assay shows addition of Orb2 oligomer increases polyA tail length in vitro. Actin serves as a control for total mRNA used. (G) Expression of Orb2A320 and Orb3A320Δ8 but not Orb2A 320Δ80 increases Tequila mRNA polyA tail length. Actin serves as amount of mRNA used in the reactions.