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. 2015 Dec 7;3:e1468. doi: 10.7717/peerj.1468

Figure 2. Production of lauric acid by engineered R. eutropha.

Figure 2

(A) Total free fatty acids (FFA) produced by ReH16 grown on rich broth (RB) and expressing UcFatB2 from plasmids (top row) of different predicted RBS strengths (RBS Calculator; bottom row). FFAs were detected in culture supernatant 48 h after arabinose induction by the fatty acid half-micro assay (Methods). Negligible amounts of free fatty acid (0.04 ± 0.06 mg/L) were detected for ReH16 expressing pJC025. (B). Chain length distribution of saturated fatty acids extracted from total culture of ReH16-pJC024 at 48 h post-induction via GCMS. For all experiments in this figure, error bars represent the standard error of the mean (S.E.M.) from N ≥ 3 independent experiments.