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. 1993 Jun 15;90(12):5529–5533. doi: 10.1073/pnas.90.12.5529

Genetic evidence for replication enhancement from a distance.

M L Gennaro 1
PMCID: PMC46754  PMID: 8516296

Abstract

Rolling circle replication of the Staphylococcus aureus plasmid pT181 requires interaction of the RepC initiator protein with the origin of replication (the ori site). A second site named cmp, which is distant from ori, is thought to stimulate replication, since a mutant pT181 plasmid lacking cmp cannot coexist with a cmp+ wild-type plasmid. Second-site mutations compensating for the loss of cmp were shown to map in repC. The compensatory mutations produced RepC proteins that, unlike the wild-type, either failed to discriminate between cmp+ and cmp- plasmids or preferred cmp- to cmp+ plasmids. These studies demonstrate that cmp stimulates the interaction of the replication initiator protein with the origin and therefore enhances DNA replication from a distance.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baker T. A., Kornberg A. Transcriptional activation of initiation of replication from the E. coli chromosomal origin: an RNA-DNA hybrid near oriC. Cell. 1988 Oct 7;55(1):113–123. doi: 10.1016/0092-8674(88)90014-1. [DOI] [PubMed] [Google Scholar]
  2. DePamphilis M. L. Transcriptional elements as components of eukaryotic origins of DNA replication. Cell. 1988 Mar 11;52(5):635–638. doi: 10.1016/0092-8674(88)90398-4. [DOI] [PubMed] [Google Scholar]
  3. Gennaro M. L., Iordanescu S., Novick R. P., Murray R. W., Steck T. R., Khan S. A. Functional organization of the plasmid pT181 replication origin. J Mol Biol. 1989 Jan 20;205(2):355–362. doi: 10.1016/0022-2836(89)90346-x. [DOI] [PubMed] [Google Scholar]
  4. Gennaro M. L., Novick R. P. An enhancer of DNA replication. J Bacteriol. 1988 Dec;170(12):5709–5717. doi: 10.1128/jb.170.12.5709-5717.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gennaro M. L., Novick R. P. cmp, a cis-acting plasmid locus that increases interaction between replication origin and initiator protein. J Bacteriol. 1986 Oct;168(1):160–166. doi: 10.1128/jb.168.1.160-166.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Greenstein D., Horiuchi K. Replication enhancer-independent mutation increases the co-operativity with which an initiator protein binds its origin. J Mol Biol. 1990 Jan 5;211(1):91–101. doi: 10.1016/0022-2836(90)90013-C. [DOI] [PubMed] [Google Scholar]
  7. Greenstein D., Zinder N. D., Horiuchi K. Integration host factor interacts with the DNA replication enhancer of filamentous phage f1. Proc Natl Acad Sci U S A. 1988 Sep;85(17):6262–6266. doi: 10.1073/pnas.85.17.6262. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Iordanescu S. Specificity of the interactions between the Rep proteins and the origins of replication of Staphylococcus aureus plasmids pT181 and pC221. Mol Gen Genet. 1989 Jun;217(2-3):481–487. doi: 10.1007/BF02464921. [DOI] [PubMed] [Google Scholar]
  9. Iordanescu S. Staphylococcus aureus chromosomal mutation specifically affecting the copy number of Inc3 plasmids. Plasmid. 1983 Sep;10(2):130–137. doi: 10.1016/0147-619x(83)90065-3. [DOI] [PubMed] [Google Scholar]
  10. Iordănescu S., Surdeanu M. Complementation of a plasmid replication defect by autonomous incompatible plasmids in Staphylococcus aureus. Plasmid. 1980 Jul;4(1):1–7. doi: 10.1016/0147-619x(80)90078-5. [DOI] [PubMed] [Google Scholar]
  11. Iordănescu S. Temperature-sensitive mutant of a tetracycline resistance staphylococcal plasmid. Arch Roum Pathol Exp Microbiol. 1976 Jul;35(3):257–264. [PubMed] [Google Scholar]
  12. Khan S. A., Novick R. P. Complete nucleotide sequence of pT181, a tetracycline-resistance plasmid from Staphylococcus aureus. Plasmid. 1983 Nov;10(3):251–259. doi: 10.1016/0147-619x(83)90039-2. [DOI] [PubMed] [Google Scholar]
  13. Khoury G., Gruss P. Enhancer elements. Cell. 1983 Jun;33(2):313–314. doi: 10.1016/0092-8674(83)90410-5. [DOI] [PubMed] [Google Scholar]
  14. Klippel A., Cloppenborg K., Kahmann R. Isolation and characterization of unusual gin mutants. EMBO J. 1988 Dec 1;7(12):3983–3989. doi: 10.1002/j.1460-2075.1988.tb03286.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Lange-Gustafson B. J., Nash H. A. Purification and properties of Int-h, a variant protein involved in site-specific recombination of bacteriophage lambda. J Biol Chem. 1984 Oct 25;259(20):12724–12732. [PubMed] [Google Scholar]
  16. Mukherjee S., Erickson H., Bastia D. Enhancer-origin interaction in plasmid R6K involves a DNA loop mediated by initiator protein. Cell. 1988 Feb 12;52(3):375–383. doi: 10.1016/s0092-8674(88)80030-8. [DOI] [PubMed] [Google Scholar]
  17. Nordström K., Molin S., Light J. Control of replication of bacterial plasmids: genetics, molecular biology, and physiology of the plasmid R1 system. Plasmid. 1984 Sep;12(2):71–90. doi: 10.1016/0147-619x(84)90054-4. [DOI] [PubMed] [Google Scholar]
  18. Novick R. P. Genetic systems in staphylococci. Methods Enzymol. 1991;204:587–636. doi: 10.1016/0076-6879(91)04029-n. [DOI] [PubMed] [Google Scholar]
  19. Novick R. P. Plasmid incompatibility. Microbiol Rev. 1987 Dec;51(4):381–395. doi: 10.1128/mr.51.4.381-395.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Polak J., Novick R. P. Closely related plasmids from Staphylococcus aureus and soil bacilli. Plasmid. 1982 Mar;7(2):152–162. doi: 10.1016/0147-619x(82)90074-9. [DOI] [PubMed] [Google Scholar]
  21. Projan S. J., Monod M., Narayanan C. S., Dubnau D. Replication properties of pIM13, a naturally occurring plasmid found in Bacillus subtilis, and of its close relative pE5, a plasmid native to Staphylococcus aureus. J Bacteriol. 1987 Nov;169(11):5131–5139. doi: 10.1128/jb.169.11.5131-5139.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Rawlins D. R., Milman G., Hayward S. D., Hayward G. S. Sequence-specific DNA binding of the Epstein-Barr virus nuclear antigen (EBNA-1) to clustered sites in the plasmid maintenance region. Cell. 1985 Oct;42(3):859–868. doi: 10.1016/0092-8674(85)90282-x. [DOI] [PubMed] [Google Scholar]
  23. Reisman D., Yates J., Sugden B. A putative origin of replication of plasmids derived from Epstein-Barr virus is composed of two cis-acting components. Mol Cell Biol. 1985 Aug;5(8):1822–1832. doi: 10.1128/mcb.5.8.1822. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Scott J. R. Regulation of plasmid replication. Microbiol Rev. 1984 Mar;48(1):1–23. doi: 10.1016/b978-0-12-048850-6.50006-5. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Walker S. S., Francesconi S. C., Eisenberg S. A DNA replication enhancer in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1990 Jun;87(12):4665–4669. doi: 10.1073/pnas.87.12.4665. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Wang P. Z., Projan S. J., Henriquez V., Novick R. P. Specificity of origin recognition by replication initiator protein in plasmids of the pT181 family is determined by a six amino acid residue element. J Mol Biol. 1992 Jan 5;223(1):145–158. doi: 10.1016/0022-2836(92)90722-v. [DOI] [PubMed] [Google Scholar]
  28. Wang P. Z., Projan S. J., Leason K. R., Novick R. P. Translational fusion with a secretory enzyme as an indicator. J Bacteriol. 1987 Jul;169(7):3082–3087. doi: 10.1128/jb.169.7.3082-3087.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]

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