Draft Genome Sequence of Bacillus marisflavi TF-11^T (JCM 11544), a Carotenoid-Producing Bacterium Isolated from Seawater from a Tidal Flat in the Yellow Sea

Abstract

Bacillus marisflavi TF-11^T (JCM 11544) is a Gram-positive, spore-forming, and carotenoid-producing bacterium isolated from seawater from a tidal flat in the Yellow Sea. Here, we report the first draft genome sequence of B. marisflavi TF-11^T, which comprises 4.31 Mb in 11 scaffolds with a G+C content of 48.57%.

GENOME ANNOUNCEMENT

The type strain TF-11^T of Bacillus marisflavi, a Gram-positive or Gram-variable (in older cultures), endospore-forming, and moderately halophilic bacterium, was isolated from seawater from a tidal flat in the Yellow Sea in South Korea (1). So far, the different strains of B. marisflavi have been found in a variety of environments, such as rice paddy soils (2), hypersaline microbial mats (3), the solid waste and liquid effluent of an electroplating industry (4), a tropical soil contaminated with petroleum hydrocarbons (5), and different plant rhizospheres (6).

It was uncovered that B. marisflavi produces carotenoid with absorption maxima at 455 nm (7), a finding consistent with the pale yellow phenotype of its colonies (1). Some strains of B. marisflavi, which inhabit varied plant rhizospheres, displayed the ability to fix nitrogen (2, 6). In environmental biotechnology, B. marisflavi was found to be one of the main three members in a lubricant-degrading microbial consortia (5); B. marisflavi tolerated chromium concentrations up to 700 mg/liter and performed chromium biosorption quickly and efficiently (4). In biomedical applications, the Bacillus marisflavi biomass was used as a reducing and stabilizing agent for the preparation of water-soluble grapheme, which exhibits obvious cytotoxicity against human breast cancer cells (8). Here, we report the draft genome of B. marisflavi TF-11^T, the first released B. marisflavi genome sequence.

The genome sequence of B. marisflavi TF-11^T was obtained by paired-end sequencing on the Illumina Hiseq 2500 system. Two different DNA libraries with insert sizes of 500 and 5,000 bp were constructed and sequenced. After filtering of the 1.21-Gb raw data, the 1.19-Gb clean sequence data were obtained, providing approximately 150-fold coverage. The average DNA G+C content was 48.57%, being in accordance with the previously acquired value of 49 mol% acquired by HPLC determination (1). The reads were assembled via SOAPdenovo software version 2.04 (9). Through the data assembly, 11 scaffolds consisting of 4,312,088 bp were obtained, and the scaffold N₅₀ was 1,039,146 bp. The average length of the scaffolds was 392,008 bp, and the longest and shortest scaffolds were 1,799,863 bp and 1,254 bp, respectively. Moreover, 92.09% clean reads were aligned back to the genome, by which 99.91% of the genome sequence was covered.

The assembly was uploaded for annotation to the National Center for Biotechnology Information Prokaryotic Genomes Annotation Pipeline version 2.9 (PGAAP) (http://www.ncbi.nlm.nih.gov/genomes/static/Pipeline.html). A total of 4,340 genes were predicted, including 4,143 coding sequences (CDS), 106 pseudogenes, and 84 tRNA and 6 rRNA genes. There were 3,185 and 2,167 genes assigned with COG and KEGG databases, respectively.

Nucleotide sequence accession numbers.

This whole-genome shotgun project has been deposited at DDBJ/EMBL/GenBank under the accession number LGUE00000000. The version described in this paper is version LGUE01000000.