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Cleavage of 32P-labeled AP- and F-substrates by the mutant APE1. Top panel, analysis by 20% PAGE; autoradiograms are shown. Reaction mixtures (10 μL) contained 3 μM substrate and 3 μM enzyme. Bottom panel, time course of the cleaved product accumulation; ●, AP-substrate incision; ○, F-substrate incision. Error bars reflect the standard error of the mean from three independent experiments.
