Table 2.

Rate Constants for Interactions of Wild-Type APE1 with AP-, F-, and G-Containing DNA

rate constants	substrate (ligand) X
	AP	F	G
k1X (M −1 s −1)	(8.93 ± 0.48) × 107	(2.0 ± (0.1) × 108	(7.4 ± (0.5) × 10
k−1X (s −1)	6.02 ± 0.69	14.6 ± 0.21	17.4 ± (0.6
k2X (s −1)	3.24 ± 0.07	5.61 ± 0.15	0.17 ± (0.01
k−2X (s −1)	1.83 ± 0.05	28.6 ± 0.32	0.76 ± (0.02
k3X (s −1)		6.55 ± 0.18
k−3X (s −1)		0.15 ± 0.01
kirrX (s −1)	3.20 ± 0.06	2.05 ± 0.04
KdX (M)	(0.96 ± 0.05) × 10−6	(5.47 ± 0.11) × 10−6
KaX (M−1)a	(4.11 ± 0.08) × 107	(1.33 ± 0.06) × 108	(5.2 ± (0.2) × 104a
KtitrWT (M)		(8.31 ± 0.10) × 10−7

^aEquilibrium association constant K_a was calculated from the equation $K_a={\displaystyle \sum _{i=1}^N}{\displaystyle \prod _{j=1}^{j=i}}{K}_j$ for N-step binding (see Scheme 1). $K_j=\frac{k_i}{k_{-j}}$.