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. 1993 Jun 15;90(12):5643–5647. doi: 10.1073/pnas.90.12.5643

Noninvolvement of the long terminal repeat of transposable element 17.6 in insecticide resistance in Drosophila.

J M Delpuech 1, C F Aquadro 1, R T Roush 1
PMCID: PMC46777  PMID: 8390673

Abstract

Waters and colleagues recently suggested [Waters, L. C., Zelhof, A. C., Shaw, B. J. & Ch'ang, L.-Y. (1992) Proc. Natl. Acad. Sci. USA 89, 4855-4859] that an insertion of a long terminal repeat of transposable element 17.6 into the 3' untranslated region of a P450 gene leads to susceptibility to the insecticide DDT in Drosophila melanogaster. We tested this hypothesis by screening lines from around the world and found that the presence or absence of a long terminal repeat was uncorrelated with resistance in 31 strains of D. melanogaster and Drosophila simulans. Thus we must reject the hypothesis that the insertion of a long terminal repeat leads to DDT susceptibility in Drosophila.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aquadro C. F., Lado K. M., Noon W. A. The rosy region of Drosophila melanogaster and Drosophila simulans. I. Contrasting levels of naturally occurring DNA restriction map variation and divergence. Genetics. 1988 Aug;119(4):875–888. doi: 10.1093/genetics/119.4.875. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brattsten L. B., Holyoke C. W., Jr, Leeper J. R., Raffa K. F. Insecticide resistance: challenge to pest management and basic research. Science. 1986 Mar 14;231(4743):1255–1260. doi: 10.1126/science.231.4743.1255. [DOI] [PubMed] [Google Scholar]
  3. Dapkus D. Genetic localization of DDT resistance in Drosophila melanogaster (Diptera: Drosophilidae). J Econ Entomol. 1992 Apr;85(2):340–347. doi: 10.1093/jee/85.2.340. [DOI] [PubMed] [Google Scholar]
  4. Dapkus D., Merrell D. J. Chromosomal analysis of DDT-resistance in a long-term selected population of Drosophila melanogaster. Genetics. 1977 Dec;87(4):685–697. doi: 10.1093/genetics/87.4.685. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Devereux J., Haeberli P., Smithies O. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387–395. doi: 10.1093/nar/12.1part1.387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ffrench-Constant R. H., Roush R. T. Gene mapping and cross-resistance in cyclodiene insecticide-resistant Drosophila melanogaster (Mg.). Genet Res. 1991 Feb;57(1):17–21. doi: 10.1017/s0016672300028986. [DOI] [PubMed] [Google Scholar]
  7. Houpt D. R., Pursey J. C., Morton R. A. Genes controlling malathion resistance in a laboratory-selected population of Drosophila melanogaster. Genome. 1988 Dec;30(6):844–853. doi: 10.1139/g88-136. [DOI] [PubMed] [Google Scholar]
  8. Hällström I. Genetic regulation of the cytochrome P-450 system in Drosophila melanogaster. II. Localization of some genes regulating cytochrome P-450 activity. Chem Biol Interact. 1985 Dec 31;56(2-3):173–184. doi: 10.1016/0009-2797(85)90004-3. [DOI] [PubMed] [Google Scholar]
  9. Rubin G. M., Brorein W. J., Jr, Dunsmuir P., Flavell A. J., Levis R., Strobel E., Toole J. J., Young E. Copia-like transposable elements in the Drosophila genome. Cold Spring Harb Symp Quant Biol. 1981;45(Pt 2):619–628. doi: 10.1101/sqb.1981.045.01.080. [DOI] [PubMed] [Google Scholar]
  10. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Shepanski M. C., Kuhr R. J., Glover T. J. Resistance of Drosophila melanogaster to DDT. J Econ Entomol. 1977 Oct;70(5):539–543. doi: 10.1093/jee/70.5.539. [DOI] [PubMed] [Google Scholar]
  12. Waters L. C., Zelhof A. C., Shaw B. J., Ch'ang L. Y. Possible involvement of the long terminal repeat of transposable element 17.6 in regulating expression of an insecticide resistance-associated P450 gene in Drosophila. Proc Natl Acad Sci U S A. 1992 Jun 1;89(11):4855–4859. doi: 10.1073/pnas.89.11.4855. [DOI] [PMC free article] [PubMed] [Google Scholar]

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