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. Author manuscript; available in PMC: 2015 Dec 14.
Published in final edited form as: Yeast. 2010 Aug 24;28(1):1–8. doi: 10.1002/yea.1814

Figure 2.

Figure 2

Detection of ADE2 dsRNA by RT-PCR. Total RNA from EAGA (lanes 2, 3, 6 and 7) and the parental strain, CAI4 (lanes 4, 5, 8 and 9), was reverse-transcribed and used in PCRs with a single oligonucleotide of ADE2 sequences (ADE2-35; lanes 2, 3, 4, and 5). A PCR amplicon was detected only in reactions with RT templates from EAGA in addition to a faster migrating band probably due to secondary structure (hairpin) of the inverted repeat DNA product (lane 2). Controls: no RT reactions to exclude DNA contamination (lanes 3, 5, 7 and 9), and the amplification of an actin amplicon (lanes 6, 7, 8 and 9) that served as positive control for the RT-PCR. M, DNA size markers (HyperLadder IV, Bioline). The migration of the 1000 and 500 bp bands are indicated on the left