Skip to main content
. Author manuscript; available in PMC: 2015 Dec 14.
Published in final edited form as: Yeast. 2010 Aug 24;28(1):1–8. doi: 10.1002/yea.1814

Figure 3.

Figure 3

Cell extract processing of blunt and hairpin dsRNA templates. A hairpin (ADE2-GFP-ADE2) or blunt (ADE2 sequences only) radiolabelled substrate was incubated with SC5314 cell extracts, and the product resolved in a 15% acrylamide, 7 M urea gel. Lanes 1, 2, 5 and 6 were incubated with the blunt dsRNA; lanes 3, 4, 7 and 8 were incubated with the hairpin dsRNA. Odd-numbered lanes lacked cell extract. The RNase reaction is dependent upon ATP hydrolysis (4 °C reactions). The migration of RNA size markers is indicated on the left (Decade Markers, Ambion/Applied Biosystems)