. Author manuscript; available in PMC: 2016 Mar 3.

Published in final edited form as: Biochemistry. 2015 Feb 19;54(8):1681–1693. doi: 10.1021/bi501171r

Table 3.

Published Results for Alanine Substitutions Performed in the gp41 MPER Region

Residue	Strains	Effect of Alanine mutation
L660A	JR2	<30% of WT infectivity⁶³
L661A	JR2	Similar infectivity to WT⁶³
E662A	JR2	Similar infectivity to WT⁶³
L663A	JR2	Similar infectivity to WT⁶³
D664A	JR2	Similar infectivity to WT⁶³
K665A	JR2	Similar infectivity to WT⁶³
W666A	JR2	<10% of WT infectivity⁶³
W666A	HXB2	Expression processing, gp120 association, and cell-cell fusion similar to WT³
S668A	JR2 (N668A)	Similar infectivity to WT⁶³
L669A	JR2	<30% of WT infectivity⁶³
W670A	JR2	>30% of WT infectivity⁶³
W670A	HXB2	Expression processing, gp120 association, and cell-cell fusion similar to WT³
N671A	JR2	Similar infectivity to WT⁶³
W672A	JR2	<30% of WT infectivity⁶³
F673A	JR2	Similar infectivity to WT⁶³
N674A	JR2	Similar infectivity to WT⁶³
I675A	JR2	<30% of WT infectivity⁶³
T676A	JR2	>30% of WT infectivity⁶³
N677A	JR2	Similar infectivity to WT⁶³
W678A	JR2	>30% of WT infectivity⁶³
	HXB2, NL4-3	Expression processing, gp120 association, and cell-cell fusion similar to WT³
	HXBc2	Processing to WT level, but slightly reduced association of gp120. Cell-cell fusion similar to WT⁶⁴
L679A	JR2	<30% of WT infectivity⁶³
W680A	JR2	>30% of WT infectivity⁶³
W680A	HXB2, NL4-3	Expression processing, gp120 association, and cell-cell fusion similar to WT³