Skip to main content
. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Methods. 2015 Aug 12;91:13–19. doi: 10.1016/j.ymeth.2015.08.008

Figure 1. Schematic of affinity purification technique.

Figure 1

Cells are infected with dengue virus at an MOI of 1. Mock infected cells serve as a negative control. RNA-protein cross-links are induced 30 hours post-infection by exposing the cells to 254 nm UV. Cells are lysed under denaturing conditions and incubated with biotinylated antisense DNA oligonucleotides. RNA-protein complexes are captured on streptavidin-coated magnetic beads. Protein can be digested with proteinase K for subsequent RNA analysis by RT-qPCR. Alternatively, proteins can be liberated from the RNA by RNase treatment and analyzed by Western blot or en masse by mass spectrometry.