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. 2015 Dec 13;17(12):871–881. doi: 10.1016/j.neo.2015.11.007

Figure 2.

Figure 2

Time-lapse imaging of EGFP and tdTomato fluorescence in control (− CHX) and CHX-treated (+ CHX) PC3-HRE-GFP/HRE-ODD-luc/tdTomato cells after 48 hours at 1% O2. The upper panel shows representative images of the evolution of EGFP and tdTomato fluorescence in control and CHX-treated cell colonies after reoxygenation. The lower panel shows the quantification of EGFP (☐) and tdTomato (•) fluorescence in the corresponding control or CHX-treated cell colonies (100 μg/ml). EGFP and tdTomato intensities were normalized to values at time zero. Data are expressed as mean ± SEM.