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. Author manuscript; available in PMC: 2016 Sep 14.
Published in final edited form as: Biomacromolecules. 2015 Aug 17;16(9):2684–2692. doi: 10.1021/acs.biomac.5b00557

Figure 5.

Figure 5

ELISA-based FGFR-binding assay. FGFR1α(IIIc) was used to coat a 96-well-plate. The wells were treated with various formulations: blank buffer, 1 ng/mL of bFGF with or without 1 µg/mL of heparin or pVS 35.9 kDa. The procedure is detailed in the Methods section. The graphed absorbance was the difference between the signals at λ = 450 nm and the background signals at λ = 630 nm. The experiment was repeated two times, each had n = 3. Error bars represent STDEV. Statistical analysis was performed using Student’s t-test; * p < 0.05 for the ‘bFGF (+) heparin’ and ‘bFGF (+) pVS’ group compared to the control group ‘bFGF’.