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. 1993 Jul 1;90(13):6213–6217. doi: 10.1073/pnas.90.13.6213

Complex formation between RAS and RAF and other protein kinases.

L Van Aelst 1, M Barr 1, S Marcus 1, A Polverino 1, M Wigler 1
PMCID: PMC46898  PMID: 8327501

Abstract

We used a Saccharomyces cerevisiae genetic system to detect the physical interaction of RAS and RAF oncoproteins. We also observed interaction between RAS and byr2, a protein kinase implicated as a mediator of the Schizosaccharomyces pombe ras1 protein. Interaction with RAS required only the N-terminal domains of RAF or byr2 and was disrupted by mutations in either the guanine nucleotide-binding or effector-loop domains of RAS. We observed interaction between MEK (a kinase that phosphorylates mitogen-activated protein kinases) and the catalytic domain of RAF. RAS and MEK also interacted but only when RAF was overexpressed.

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Selected References

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