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. 1993 Jul 1;90(13):6340–6344. doi: 10.1073/pnas.90.13.6340

A hairpin ribozyme inhibits expression of diverse strains of human immunodeficiency virus type 1.

M Yu 1, J Ojwang 1, O Yamada 1, A Hampel 1, J Rapapport 1, D Looney 1, F Wong-Staal 1
PMCID: PMC46924  PMID: 8327516

Abstract

Ribozymes have enormous potential as antiviral agents. We have previously reported that a hairpin ribozyme expressed under the control of the beta-actin promoter that cleaves human immunodeficiency virus type 1 (HIV-1) RNA in the leader sequence can inhibit HIV-1 (pHXB2gpt) expression. For such a ribozyme in a retroviral vector delivery system to be useful in gene therapy for the treatment of HIV-1 infection, it must be able to inhibit the expression of multiple HIV-1 strains. We have now cloned this ribozyme into various regular expression vectors (including retroviral vectors) by using various gene expression control strategies. Here we show by transient transfection that inhibition of expression of diverse strains of HIV-1 can be achieved by this ribozyme expressed in the proper vectors. These data further support the potential of this hairpin ribozyme as a therapeutic agent for HIV-1.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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