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. 2015 Oct 30;4(4):752–763. doi: 10.3390/pathogens4040752

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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Msb2 shed by C. albicans does not always protect cells from Hst 5. Hst 5 susceptibility of various C. albicans Msb2 domain mutant cells was tested and compared with the levels of Msb2 shedding (shown in slot blot above). All strains have Hst 5 killing similar to WT except TM-CYTΔ that showed significant reduction in Hst 5 activity. msb2Δ cells with no shedding only showed a slight reduction in killing. CAI-4 cells with untagged WT Msb2 were used as the control. Statistical analysis was performed using student t-test; significance at p < 0.05.