Figure 5.

Transforming growth factor‐β (TGF‐β) ‐exposed antigen‐presenting cells (APCs) deficient in thrombospondin‐1 (TSP‐1) or its receptor CD36 fail to induce Treg cells in vivo. Foxp3.mRFP mice (n = 3/group) were injected intravenously with (a) no APCs or ovalbumin (OVA) ‐pulsed, untreated, or TGF‐β‐treated (b) wild‐type (WT), (c) TSP‐1^−/− or (d) CD36^−/− macrophages (10⁴ cells/mouse) 12 days before determining the proportion of Foxp3‐expressing CD4 T cells in their spleens. The plots show analysis of pooled splenocytes harvested from mice in each group. Gates in each experiment were set based on respective untreated APC controls and in some include CD4 T cells expressing lower levels of Foxp3. The data represent three individual experiments.