Figure 5.

Effect of NaCl and KNO₃/NaCl on promoter activity, transcription, and translation of OpdQ in Pseudomonas aeruginosa PAO1 and clinical strains grown in 2% oxygen. Luciferase transcription levels were relative to clone G (not shown) for cultures grown in 2% oxygen +/− NaCl (A and D). opdQ transcription was normalized to rpoD transcription (B and E) and the amount of OpdQ was normalized to total protein (C and F). The amount of total protein used for each strain was the same as in Figure 2. Experiments were performed with at least three independent biological cultures incubated at 37°C in a hypoxic chamber (2% O₂, 5% CO₂, and 93% N₂). Immunoblot images are representative of samples on the same membrane. Error bars indicate standard deviation. Significantly different from Mueller–Hinton broth in 2% oxygen; *P < 0.05, **P < 0.01, ***P < 0.001.