Figure 4. Gene silencing of DR5 suppressed growth inhibition induced by combined treatment with α-mangostin and rTRAIL.

A. TRAIL-sensitive and -resistant DLD-1 cells were transfected with control or DR5 siRNA (2 nM) plasmids for 48 h and then treated with α-mangostin (7 μM) and/or rTRAIL (5 ng/ml) for 48 h. Western blot analysis was performed to determine the expression of DR5, and β-actin was used as an internal control.