Figure 4. Tumorigenic potential of high passage mucoepidermoid carcinoma cells sorted for ALDH/CD44.

A. Graph depicting the volume of tumors generated by the transplantation of FACS-sorted UM-HMC-3B cells (ALDH^highC44^high or ALDH^lowCD44^low) in immunodeficient mice. 5,000 sorted UM-HMC-3B cells (passage 103) and 900,000 endothelial (HDMEC) cells were seeded on biodegradable scaffolds and transplanted into the subcutaneous space of SCID mice. Tumors were measured weekly and mice were euthanized once the tumors reached 700-1,500 mm³. B. Kaplan-Meyer analysis of time to palpability of tumors generated with ALDH^highCD44^high or ALDH^lowCD44^low cells. Tumors were considered palpable once they reached 200 mm³. C. Regression analysis of growth after palpability (200 mm³) of tumors generated with FACS-sorted ALDH^highCD44^high or ALDH^lowCD44^low cells. D. H&E staining of tumors generated with FACS-sorted ALDH^highCD44^high and ALDH^lowCD44^low cells. Images were taken at 40X and 100X. E. Graph depicting the volume of tumors generated by the transplantation of FACS-sorted UM-HMC-3B cells (ALDH^highC44^high or ALDH^high) in immunodeficient mice. 5,000 sorted UM-HMC-3B cells (passage 104) and 900,000 endothelial (HDMEC) cells were seeded on biodegradable scaffolds and transplanted into the subcutaneous space of SCID mice. Tumors were measured weekly and mice were euthanized once the tumors reached 700-1,500 mm³. F. Kaplan-Meyer analysis of time to palpability of tumors generated with ALDH^highC44^high or ALDH^high cells.