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. 2015 Sep 10;6(29):26690–26701. doi: 10.18632/oncotarget.5614

Figure 3. Changes in levels of mitochondrial cytochrome C oxidase III proteins in the ageing retina of C57BL/6 mice.

Figure 3

A. Retinal sections of both young and old mice immunostained for cytochrome C oxidase III (COX III) antibody (red) and nuclei counterstain with 4′, 6-diamino-2-phenylindole (DAPI) (Blue). COX III strongly labelled photoreceptor inner segments in the young retina. B. The expression of COX III in the outer plexiform layer is significantly stronger in young mice compared to old ones (P = 0.0040). C. Measurement of COX III expression in the inner segment of the photoreceptor of young and old mice. There is a significant decrease in the expression of COX III in inner segment of the old retina than in the young retina (P = 0.0040). D. Western blot analysis of COX III in young and old mice showing a significant decrease in the retina of old mice compared to young (P = 0.0349). Mean ± SEM. Scale bar = 25μm. GCL; ganglion cell layer, IPL; Inner plexiform layer, INL; inner nuclear layer, OPL; outer plexiform layer, ONL; outer nuclear layer, PR; photoreceptor layer and RPE; retinal pigment epithelial.