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. 2015 Aug 11;6(29):27130–27145. doi: 10.18632/oncotarget.4825

Figure 5. The co-regulation of hTERT promoter activity, hTERT expression, and cell proliferation in H1299 cells with overexpression of CBP and low expression of RFPL3 or simultaneous overexpression of CBP and RFPL3.

Figure 5

A. H1299 cells were co-transfected with CBP plasmid or Lac Z plasmid, and RFPL3-specific siRNA (siRFPL3-1 and siRFPL3-2) or non-specific siRNA (NSP) and the plasmids of hTERT promoter driven-luciferase. 48 hours later, the relative luciferase intensity per μg protein was calculated in the treated cells. B. H1299 cells were co-transfected with CBP plasmid and RFPL3-specific siRNAs (siRFPL3-1 and siRFPL3-2) or non-specific siRNA (NSP). 48 hours after treatment, the expressions of hTERT, RFPL3 and CBP proteins in the lung cancer cell extracts were examined by western blot. C. Cell viability of these cells treated as above was assayed through MTT assay. D. H1299 cells stably overexpressing RFPL3 were co-transfected with CBP plasmid or Lac Z plasmid and the plasmids of hTERT promoter driven-luciferase. 48 hours later, the relative luciferase intensity per μg protein was calculated. E. H1299 cells with stable overexpression of RFPL3 were co-transfected with CBP plasmid or Lac Z plasmid. 48 hours after treatment, the expressions of hTERT, RFPL3 and CBP proteins in the lung cancer cells were examined by western blot. F. Cell viability was measured by MTT assay in H1299 cells with both overexpression of CBP and RFPL3 simultaneously.