Skip to main content
. 2015 Aug 6;6(29):27214–27226. doi: 10.18632/oncotarget.4877

Figure 3. Establishment and characterization of DU145 cells stably overexpressing miR-17/106a.

Figure 3

A. PTEN mRNA (left) and protein (right) were significantly decreased in miR-17/106a MIMIC compared to EV MIMIC cells. PTEN mRNA expression was detected by real time PCR and protein was detected by western blot. B. Resveratrol inhibits relative abundance of miRs-17 and -106a in EV and miR-17/106a MIMIC cells as detected by real time PCR. C, D. Resveratrol and pterostilbene enhanced expression of PTEN mRNA (C) and protein (D) in EV MIMIC and miR-17/106a MIMIC cells. Fold change in expression of miRNAs and mRNA was calculated by the 2−ΔΔCt method. Data represent the mean ± SEM from at least three independent experiments. Quantitation of blots was performed using Image J software. Comparisons between non-transfected and miR transfected samples (#) and vehicle and compound-treated samples (*) are shown. #p < 0.05; ##p < 0.01; *p < 0.05; **p < 0.01; ***p < 0.001. EV, Empty vector, Ctrl, Control, Res, resveratrol; Pter, pterostilbene.