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. 2015 Jul 25;6(29):27312–27331. doi: 10.18632/oncotarget.4743

Figure 1. Characterization of DOX-inducible MIA PaCa-2 cell lines.

Figure 1

A. qPCR analysis of HuR mRNA expression in Mia.CTRL, Mia.sh290, and Mia.sh700 cells treated with 0 or 2 μg/ml DOX for 5 days (left), and Mia.EV and Mia.HuR cells treated with 0 or 2 μg/ml DOX for 2 days (right), normalized to 18S rRNA expression. Mia.sh290 and Mia.sh700 are inducible HuR-silencing cell lines, whereas Mia.HuR is an inducible HuR-overexpressing cell line. Mia.CTRL and Mia.EV are the respective control cell lines. B. Western blotting analysis of HuR protein expression in Mia.CTRL, Mia.sh290, and Mia.sh700 cells treated with 0 or 2 μg/ml DOX for 5 days (left), and Mia.EV and Mia.HuR cells treated with 0 or 2 μg/ml DOX for 2 days (right), normalized to alpha-tubulin protein expression. ns = non-significant; *** = p < 0.001; **** = p < 0.0001.