Figure 7. p53 independent apoptosis is a general response to UBF loss in an oncogenic stress context.

A. Ubf^fl/fl/Er-cre^+/+/Sv-T/p53^−/− and their counterpart B. Ubf^fl/fl/Er-cre^+/+/Ras/p53^−/− and C. Ubf^fl/fl/Er-cre^+/+/Ras/Myc/p53^−/− iMEFs cells were subjected to a TUNEL reaction immediately before and at several time points after treatment with 4-HT. All cells synchronously became TUNEL positive at 96 h post 4-HT, while neither effect was observed 24 h previously. Recombination of the Ubf gene and UBF protein levels were assayed in parallel and closely followed those shown in Figure S2B and S2C.