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. 2015 Aug 7;6(29):27628–27640. doi: 10.18632/oncotarget.4733

Figure 3. N-terminus of CASZ1b interacts with NuRD subunits and zinc finger 3 is required for DNA repair proteins binding.

Figure 3

A. Cartoon of CASZ1b mutant constructs (ZF represents zinc finger), to the right of the cartoon, the relative transcriptional activity of CASZ1b mutants compared to wild type CASZ1b is shown. B. EV, FLAG-CASZ1b, or FLAG-CASZ1b mutant construct was transiently transfected into HEK293T cells, and anti-FLAG antibody was used for co-immunoprecipitation (co-IP) of the CASZ1b complex from the whole cell extracts. The co-IP products were resolved by SDS-PAGE and stained with comassie stain to show the pull down of CASZ1b and the mutant constructs, or probed for GAPDH by western blotting to show the specificity of co-IP. C. EV, FLAG-CASZ1b or the mutant construct was transiently transfected into HEK293T cells. The cell extracts were used for co-IP with anti-FLAG antibody, and probed for the subunits of NuRD by western blot. D. EV, FLAG-CASZ1b or the mutant construct was transiently transfected into HEK293T cells. The cell extracts were used for co-IP with anti-FLAG antibody, and probed for DNA repair proteins by western blot.