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. 2015 Aug 7;6(29):27628–27640. doi: 10.18632/oncotarget.4733

Figure 5. Putative poly-ADP-ribose (PAR) binding motif of CASZ1b is required for DNA repair proteins and histone H3 binding but not for NuRD subunits binding.

Figure 5

A. a. Putative PAR binding motif existed in the middle of CASZ1b; b. this motif is conserved in different species; c. generate CASZ1b PAR binding motif mutant constructs. B. EV, FLAG-CASZ1b or the PAR binding mutant construct was transiently transfected into HEK293T cells. The cell extracts were used for co-IP with anti-FLAG antibody, and probed for PAR and PARP1 by western blot. C. Cartoon of CASZ1b N-terminus and PAR binding mutant constructs (ZF represents zinc finger), to the right of the cartoon, the transcriptional activity of CASZ1b mutants compared to wild type CASZ1b is shown. D. EV, FLAG-CASZ1b N-terminus mutant or PAR binding mutant construct was transiently transfected into HEK293T cells. The cell extracts were used for co-IP with anti-FLAG antibody, and probed for NuRD subunits, DNA repair proteins and histone H3 by western blot.