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. 2015 Jun 19;6(27):23548–23560. doi: 10.18632/oncotarget.4556

Figure 3. Hyperglycemia inhibits the effects of phenformin on cell viability and AMPK activation.

Figure 3

A. MTT assay of HeyA8 cells treated with 0-100 μM phenformin in normoglycemic (5.5 mM glucose) or hyperglycemic (25 mM glucose) conditions for 72 h. Values are mean ± SEM, n = 3. *p < 0.01 vs. same phenformin concentration in 25 mM glucose. B. MTT assay of HeyA8 cells treated with 25 μM phenformin for 72 h in the presence of increasing glucose concentrations (5.5-25 mM glucose). Values are mean ± SEM, n = 5. *p < 0.01 vs. treatment in 5.5 mM glucose. C. Western blot of pAMPK Thr172 (62 kDa), AMPK (62 kDa), and β-actin (42 kDa) in HeyA8 cells treated with 100 μM phenformin or 1 mM metformin in normoglycemic or hyperglycemic conditions for 24 h.