Laparoscopically assisted colorectal surgery provides better short-term clinical and inflammatory outcomes compared to open colorectal surgery

Abstract

Introduction

Changes in immune function after surgery may influence overall outcome, length of hospital stay, susceptibility to infection and perioperative tumour dissemination in cancer patients. Our aim was to elaborate on postoperative differences in the immune status and the intensity of the systemic inflammatory response between two groups of prospectively enrolled patients with colorectal cancer, namely patients undergoing laparoscopically assisted or open colorectal surgery.

Material and methods

Blood samples from 77 patients were taken before surgery and then 3 h, 24 h and 4 days after surgery. The inflammatory response was determined by leukocyte counts, erythrocyte sedimentation rate (ESR), C-reactive protein (CRP) and procalcitonin levels (PCT). Immune status was determined by phenotypic analysis of lymphocyte populations and the activation of mononuclear cells. CD64 expression and cytokine expression were also determined.

Results

Patients undergoing laparoscopically assisted surgery had less intraoperative blood loss (p = 0.002), earlier resumption of diet (p = 0.002) and shorter hospital stay (p = 0.02). Numbers of total leukocytes (p = 0.12), CRP (p = 0.002) and PCT (p = 0.23) were remarkably higher 4 days after surgery in patients who underwent an open colorectal procedure. There was an important decrease in monocyte HLA-DR expression 3 h after surgery in patients undergoing laparoscopically assisted surgery (p = 0.03).

Conclusions

Our study suggests that minimally invasive surgery provides better short-term clinical outcomes for patients with resectable colorectal cancer. The acute inflammatory response is less pronounced. Post-surgical immunological disturbance in both groups is similar, but we observed a divergent effect of different surgical approaches on the expression of HLA-DR on monocytes. However, our results corroborate the results of previous studies.

Introduction

Colorectal cancer remains one of the most commonly diagnosed malignancies worldwide. Surgical therapy, often combined with radiotherapy and chemotherapy, still provides the best outcomes for these patients and therefore remains the treatment of choice. Minimally invasive procedures are gaining more recognition and have developed into good alternative approaches to conventional open surgery, even in cancer surgery [1, 2]. There has been extensive research in the past decade comparing laparoscopically assisted surgery (LAS) and conventional open colorectal surgery (OCS), mainly observing short-term clinical benefits, faster recovery and increased patients’ postoperative quality of life following minimally invasive procedures [3–7]. Studies observing long-term outcomes have shown that carcinoma recurrence rates after laparoscopic resection are no different from those after open type surgery [8–10].

It has long been known that surgery itself has important immune consequences in terms of temporary suppression of the immune system, which is shown in depleted numbers of T lymphocytes, B lymphocytes and natural killer (NK) cells [11–15]. A variety of immune parameters seem to be better preserved in minimally invasive methods compared to equivalent open surgical procedures. Following conventional surgery there is more severe temporary suppression of immune cells in innate and specific immunity [16], better preservation of neutrophil function [17], and a more pronounced increase in the total number of leukocytes [18]. Cellular cytokine production seems to be better preserved after laparoscopic procedures, while cytokine plasma levels are significantly higher after conventional surgery [18]. Some authors observed down-regulation of the T-helper cell type 1 response (a decrease in IFN-γ, TNF-α, and IL-2 production) after conventional surgery but not after a minimally invasive procedure [19]. Overall, minimally invasive surgery usually causes less immunological disturbance [18, 20, 21]. Consequently, laparoscopic surgery can be performed with faster recovery, less postoperative pain and shorter hospital stay [6]. Decreased perioperative stress may be particularly important for cancer patients, since there is a higher likelihood of tumour dissemination in the early postoperative period due to changes in the patient's immune system [22]. While most authors agree that the cell-mediated immune response is better preserved after laparoscopic surgery [21, 23, 24], others have found no difference when comparing the cellular immune response [25], number and function of NK cells or monocyte HLA-DR expression [14, 25, 26]. Wichmann et al. suggest that the nonspecific immune response is less affected by laparoscopic surgery when compared with open surgery while the specific cell-mediated immunity is equally affected [20].

Due to conflicting data, our goal was to elaborate on the immunological effects in both types of surgery and evaluate the intensity of the systemic inflammatory response in colorectal cancer patients by comparing a variety of immune parameters, further exploring the activation of mononuclear cells and the role of the early sepsis marker CD64. We hypothesised that minimally invasive surgery results in reduced postoperative immune dysfunction and a reduced postoperative systemic inflammatory response.

Material and methods

Patients and surgery

Initially 77 adult patients were included in our non-randomized prospective study. All of them met the primary inclusion criteria: they were diagnosed with primary cancer of the colon or upper third of the rectum and were treated at the Clinical Department of Abdominal Surgery, University Medical Centre Ljubljana, between June 2011 and May 2012. The study design was explained to all potential participants and it was necessary to sign an informed consent form. Patients with the following conditions were excluded from the study: emergency operation due to tumour-related intestinal obstruction or perforation, suspected intra-abdominal abscess seen on preoperative imaging studies, inflammatory bowel disease (Crohn's disease, ulcerative colitis or any other nonspecific inflammatory bowel disease), previously or synchronously diagnosed malignant disease, advanced primary disease (metastatic disease or locally advanced tumour), receiving immunosuppressive therapy, or with known immunodeficiency (HIV, lymphoma, leukaemia). Patients with anastomotic leak or major postoperative intra-abdominal bleeding prior to the last evaluation (day 4 after surgery) who needed blood transfusion or surgical intervention were excluded from our study as well, since anastomotic leak or major intra-abdominal bleeding with surgical intervention is associated with much greater risk of developing a systemic inflammatory response (SIRS) and blood transfusion is associated with depression of the immune system [27, 28].

The study protocol was approved by the Commission of the Slovenian Medical Ethics Committee.

Eligible patients were consecutively enrolled in our study and they were divided into two groups. The first group of patients underwent open colorectal surgery (OCS group) and the second group laparoscopically assisted surgery (LAS group). On hospital admission, demographics, nutritional status with body mass index, presence of comorbidity factors (Table I) and primary diagnosis were recorded in all patients. In addition to data regarding the surgical procedure and postoperative course, we collected the parameters of histological findings. All patients received antibiotic prophylaxis with a single dose of gentamicin and metronidazole before the induction of anaesthesia.

Table I.

Demographic, epidemiological, surgical and pathological characteristics of enrolled patients

Variable	Surgery type
	Open (n = 34)	Laparoscopic (n = 41)	Value of p
Age [years]	67 (11.7)	63 (12.3)	0.12♣
Gender:			0.08♦
Male	13 (38)	24 (59)
Female	21 (62)	17 (41)
Final histology:			0.74♦
Adenocarcinoma	30 (88)	34 (83)
Adenomas	4 (12)	7 (17)
Staging:			0.06♦
0	0	2 (6)
I	10 (29)	13 (38)
II	11 (32)	7 (21)
III	8 (24)	11 (32)
IV	0	1 (3)
Unknown	5 (15)	0
Vascular invasion:			0.32♦
No	25 (78)	32 (80)
Yes	2 (6)	0
Lymphangiosis carcinomatosa	5 (16)	8 (20)
Perineural invasion	2 (6)	3 (8)	1♦
Comorbidities:			1♦
Arterial hypertension	14 (41)	17 (41)
Hyperlipidemia	5 (15)	7 (17)
Diabetes mellitus	2 (6)	6 (15)
Benign prostatic hypertrophy	5 (15)	2 (5)
Osteoporosis	1 (3)	1 (2)
Psoriasis	1 (3)	0
Chronic pulmonary disease	2 (6)	4 (10)
Chronic atrial fibrillation	2 (6)	7 (17)
Hemicolectomy type:			1♦
Left	11 (32)	13 (32)
Right	15 (44)	18 (44)
Anterior resection of the rectum	8 (24)	10 (24)
Number of lymph nodes	16.8 (9.0)	14.7 (9.5)	0.42♣
Resection margin status:
Closest intestinal margin	5.9 (3.1)
Radial margin	2.0 (1.1)

Results presented as n (%).

The anastomosis was performed intracorporeally in the LAS group. An extracorporeal hand-sewn or stapled anastomosis was made in patients who underwent laparoscopically assisted right hemicolectomy. The anastomosis in the open surgery group was hand-sewn except for patients who underwent low anterior resection of the rectum. Pneumoperitoneum was induced by insufflation of CO₂ and was maintained around 12 mm Hg during the entire surgical procedure.

All patients had a mechanical bowel preparation with polyethylene glycol (PEG) solution the day before surgery.

There were seven surgeons performing both types of operations. All surgeons had completed their learning curve (performing at least 100 laparoscopically assisted and open procedures) and were considered equally trained in both procedures.

Conversion to an open procedure was at the discretion of the surgeon and was defined as a need to perform an abdominal incision longer than 7 cm. Conversions to an open procedure were made because of unclear anatomical relations, laparoscopically inoperable disease, laparoscopically uncontrollable bleeding or adhesions from previous operations.

Oral food intake was also initiated at the discretion of the surgeon. Postoperative pain was objectively assessed by evaluation of the total number of days during which narcotics, administered intravenously, were needed. The length of hospital stay was defined as days after the operation until discharge home.

Perioperative mortality was defined as death occurring within 30 days of operative resection. Follow-up was obtained by physician's charts.

Blood samples

Peripheral venous blood samples were taken from all patients before surgery and then 3 h, 24 h and 4 days after surgery. The postoperative systemic inflammatory response was determined by leukocyte counts, C-reactive protein (CRP), procalcitonin (PCT) and erythrocyte sedimentation rate (ESR). Additional blood samples were collected in EDTA collection tubes to determine the immune status of the patients after surgery on the basis of phenotypic analysis of leukocyte populations and the activation of mononuclear cells by flow cytometry. Absolute numbers of T and B lymphocytes were measured, followed by determining the helper T cells (CD4+), cytotoxic T cells (CD8+), natural killer cells (CD16+CD56+), activated T cells, HLA-DR+ monocytes, CD16+CD14+ monocytes, CD16+ granulocytes and CD14+ monocytes. Lymphocytes were analysed for the expression of cell surface antigens using the following combinations of monoclonal antibodies and fluorescent labels: CD3-PE, CD4-PerCP, CD8-FITC, CD19-FITC, CD56-PE, all from Cytognos (Salamanca, Spain) and HLA-DR-PE, CD14-PerCP, CD16-FITC, all from Becton Dickinson Biosciences (Oxford, UK).

The samples were prepared by adding 10 µl of appropriate monoclonal antibodies to 50 µl of whole blood and incubating in the dark. After incubation, the erythrocytes were lysed and fixed according to the manufacturers’ instructions. The fluorescence was measured with a FACSCanto cytometer (BD Biosciences Pharmingen, San Diego, CA USA) using acquisition and analysis software. The lymphocyte populations were automatically gated. The levels of HLA-DR, CD14 and CD16 expression were assessed as their intensities, based on the arithmetic mean fluorescence intensity (MFI).

Expression of CD64 was analysed using the Leuko64 assay (Trillium Diagnostics, LLC, Maine, USA) according to the manufacturer's instructions. CD64 index measurements were derived from the ratio of linearized MFI of the cell populations and the FITC signal from the beads. An internal negative control of the assay was provided by the automated measurement of the lymphocyte CD64 index (< 1.0), while the internal positive control of the assay was provided by automated measurement of the monocyte CD64 index (> 3.0).

The serum IL-6, IL-10, IL-8, TNF, IL-1β and IL-12p70 levels were determined from sera with flow-cytometry, using the Cytometric Bead Array (Human Inflammatory Cytokine Kit, BD Biosciences Pharmingen, San Diego, CA USA).

Statistical analysis

The statistical language R was used to analyse the data (R version 3.0, R Foundation for Statistical Computing, Vienna, Austria). Mean values and standard deviations are reported. Differences between groups were tested by the Mann-Whitney test (^♣) and mixed effect linear regression model (^♠). Associations between categorical variables were tested by Fisher's exact test (^♦). Primary hypotheses (results in Figure 1) were tested at the significance level 0.05 (*). The problem of multiple comparisons within secondary hypotheses was counteracted by testing at the significance level 0.01 (**).

Figure 1.

Mean response profiles for lymphocyte subpopulation levels at baseline (before surgery), after 3 h, 1 day and 4 days after surgery, OCS group (dashed black line) and LAS (grey line)

Results

Seventy-seven adult patients, diagnosed with primary colorectal cancer, were initially enrolled in our prospective study. Two patients were excluded prior to data analysis (one due to later identified metastatic disease and one because of a severe postoperative haemorrhage). Five LAS patients were converted to open type surgery. These patients were moved to the OCS group for data analysis. The majority of patients underwent surgery due to adenocarcinoma and the rest of patients due to adenomas. All resected specimens had microscopically negative resection margins (R0 resections). Groups did not differ significantly in terms of age and gender, disease pathology, localization of the tumour, histological type, staging or the presence of vascular and perineural invasion (Table I). Short-term clinical outcomes for both groups of patients are summarised in Table II.

Table II.

Summary of differences in short-term clinical outcomes between procedures

Variable	Surgery type		Value of p
	Open (n = 34)	Laparoscopic (n = 41)
Duration of surgery [min]	101 (29)	122 (36)	0.005**♣
Blood loss during surgery [ml]	189 (159)	94 (80)	0.002**♣
Length of postoperative stay at hospital [days]	8.5 (3.1)	7.3 (1.5)	0.02♣
Resumption of oral food intake [days]	4.9 (1.0)	4.2 (0.7)	0.002**♣
Duration of postoperative use of analgesic therapy [days]	4.1 (1.3)	3.6 (1.2)	0.08♣
Postoperative complications	5 (15)	2 (5)	0.23♦

Results presented as n (%) or mean (SD).

Acute inflammatory response

The dynamics of the observed acute inflammatory response, measured by values of total leucocyte count, CRP, PCT and ESR levels, are presented in Table III. The surgery type defined groups differ significantly in CRP values (p = 0.002), although the numbers of total leukocytes, CRP and PCT were remarkably higher 4 days after surgery in patients who underwent an open colorectal procedure (7.7 × 10⁹/l, 74.1 mg/l, 0.57 µg/l (OCS) vs. 6.7 10⁹/l, 54.8 mg/l, 0.35 µg/l (LAS), respectively).

Table III.

Postoperative acute inflammatory response

Variable	Time, surgery type								Value of p♠
	t = 0–		t = 3 h		t = 24 h		t = 96 h
	Mean (SD)		Mean (SD)		Mean (SD)		Mean (SD)
	OCS	LAS	OCS	LAS	OCS	LAS	OCS	LAS
Total leucocytes (109/l)	7.0 (2.4)	6.5 (2.1)	12.5 (4.1)	11.9 (2.7)	11.2 (4.2)	10.0 (2.5)	7.7 (2.3)	6.7 (2.1)	0.12
CRP [mg/l]	13.2 (30.4)	5.7 (9.6)	14.1 (23.1)	8.8 (13.8)	109.0 (42.4)	67.9 (49.3)	74.1 (44.7)	54.8 (47.4)	0.002**
PCT [µg/l]	0.04 (0.05)	0.06 (0.05)	0.22 (0.32)	0.14 (0.19)	1.97 (3.01)	0.38 (0.54)	0.57 (0.64)	0.35 (0.99)	0.23
ESR [mm/h]	24.3 (19.6)	18.2 (19.0)	15.6 (17.5)	11.8 (12.0)	24.4 (21.7)	17.0 (18.0)	45.5 (27.0)	35.5 (20.1)	0.09

Changes in immunological parameters

All lymphocyte subsets decreased considerably after both types of surgery. Mean response profiles of lymphocyte subpopulations and CD64 index at baseline and then 3 h, 24 h and 4 days after surgery are presented in Figure 1. The between-groups test indicates that there were statistically significant differences between the OCS and the LAS group over time in the response of some lymphocyte subpopulations (activated T cells (p < 0.001), HLA-DR monocytes (p = 0.03), CD14+/CD16+ monocytes (p = 0.03)). Corresponding p-values for the effect of surgery type on responding variables are reported below interaction plots in Figure 1 and the significant values are denoted with an asterisk (*). We compared cytokine levels in both groups on the fourth day after surgery. Mean levels of observed cytokines IL-8, IL-10, IL-1b, IL-12p70 and TNF were slightly higher in the OCS group of patients (9.4, 2.2, 2.4, 2.3, 2.4 ng/ml (OCS) vs. 6.9, 1.8, 2.2, 2.1, 1.9 ng/ml (LAS), respectively), but the differences between groups were not statistically significant (Table IV).

Table IV.

Cytokine expression (ng/ml) on the fourth day after surgery

Parameter	Surgery type		Value of p♣
	Open (n = 34)	Laparoscopic (n = 41)
	Mean (SD)	Mean (SD)
IL-6	9.2 (6.7)	10.5 (11)	0.68
IL-8	9.4 (5.0)	6.9 (4.0)	0.02
IL-10	2.2 (0.7)	1.8 (0.6)	0.02
IL-1b	2.4 (0.6)	2.2 (0.8)	0.19
IL-12p70	2.3 (0.9)	2.1 (0.7)	0.27
TNF	2.4 (0.9)	1.9 (0.9)	0.02

Discussion

The aim of our prospective nonrandomised clinical study was to elucidate the differences between changes in the immune responses induced by two different surgical approaches, laparoscopically assisted and open surgery, in patients with cancer of the colon or upper third of the rectum. In agreement with many other authors, we observed short-term clinical benefits for patients undergoing minimally invasive procedures compared to patients undergoing conventional surgery [20, 21, 23]. The LAS group had significantly less blood loss during surgery (mean values 94 ml (LAS) vs. 189 ml (OCS), p = 0.002) and the length of postoperative hospital stay was shorter (mean difference 1.2 days, p = 0.02). They also had earlier resumption of oral food intake (mean resumption time 4.2 days (LAS) vs. 4.9 days (OCS), p = 0.002). Duration of postoperative analgesic therapy was shorter in the LAS group, although not statistically significantly so (mean duration 3.6 days (LAS) vs. 4.1 days (OCS), p = 0.08).

Postoperative acute inflammatory response

We observed a considerably higher postoperative systemic inflammatory response in patients after OCS compared to LAS, which was especially evident on the fourth day after surgery, with importantly higher levels of CRP and total leukocyte counts in patients after OCS, compared to the LAS group (74.1 mg/l, 7.7 × 10⁹/l (OCS) vs. 54.8 mg/l, 6.7 × 10⁹/l (LAS), respectively). These findings are consistent with many previous studies [20, 21, 23]. It seems that a more prominent postoperative inflammatory response correlates with a greater amount of tissue damage in open colorectal surgery. A proinflammatory response is necessary for the initiation of repair mechanisms after injury, which is greater following conventional procedures, so it is not quite clear if these findings have any clinical significance. However, a greater inflammatory response leads to more adhesion formation. Furthermore, if the postoperative inflammatory response is too great, it can lead to organ dysfunction, so minimally invasive procedures seem to have an advantage over open surgery.

Changes in immunological parameters

Any surgical procedure causes temporary suppression of the immune system, with depleted numbers of T and B lymphocytes and NK cells.

There was evident lymphopenia after both surgical procedures, which is consistent with other studies [12]. There were no significant differences in lymphocyte counts between the OCS and LAS group (Figure 1 A–C), although the mean values of B and T lymphocytes and total counts were lower in the OCS group 4 days after surgery (0.1, 0.85 (OCS) vs. 0.16 × 10⁹/l, 0.95 × 10⁹/l (LAS), respectively). We also confirmed lower levels of NK cells, which are involved in preventing metastasis formation [29], in both groups (Figure 1 E).

Monocytes, which express HLA-DR, are a good marker of immunoparalysis since monocytes with down-regulated HLA-DR expression are not able to respond to inflammatory stimuli or present antigens [30]. A lower expression of HLA-DR on monocytes correlates with a higher incidence of infectious diseases and sepsis in trauma patients [31]. In our study we found an unexpected decrease in HLA-DR expression, which was significantly lower in the group of patients who underwent minimally invasive surgery, compared to patients who underwent conventional surgery (Figure 1 F, p = 0.03). Our observations of monocyte activation point to seemingly more pronounced monocyte suppression after laparoscopic procedures. These results are not consistent with findings of other studies [32, 33]. However, Menges et al. demonstrated that the duration of the surgical procedure has a significant influence on the postoperative expression of HLA-DR. Irrespective of the degree of surgical trauma involved, longer procedures resulted in significantly lower monocyte HLA-DR expression than shorter operations [34]. The conventional procedures in our study were significantly shorter than laparoscopically assisted procedures. Nevertheless, our results suggest the need for further research to be able to differentiate more specifically between the effects of surgical trauma and the procedure duration on the HLA-DR monocyte populations.

Blood monocytes expressing both the CD14+ and CD16+ antigen constitute a proinflammatory subtype, and exhibit features of tissue macrophages. Four days after surgery, levels of CD14+CD16+ in the OCS group were substantially lower than in the LAS group (6.62% (OCS) vs. 10.41% (LAS), Figure 1 G, p = 0.03). Human neutrophils express a low-affinity receptor for IgG: FcRIIIb (CD16), which plays an important role in phagocytosis, cell-mediated cytotoxicity and the release of free radicals and proteolytic enzymes. A decrease in CD16 expression on granulocytes is a useful marker for disease severity in patients with sepsis [35]. The expression of CD16 on granulocytes was slightly lower in OCS patients one day after surgery (6647 mfi (OCS) vs. 8785 mfi (LAS), Figure 1 H, p = 0.07), which could also suggest some degree of immunoparalysis in these patients.

Postoperative immune disturbances, such as marked suppression in cell-mediated immunity following an excessive inflammatory response and especially depletion of HLA-DR, correlate with postoperative occurrence of sepsis and infectious complications [36, 37]. Since no differences were found in complication rates between the two groups in our study, we cannot draw any conclusions regarding the clinical significance of immunological disturbances.

According to available literature on this topic, hand-sewn or stapled anastomoses have no known effect on systemic immune function or the systemic inflammatory response after surgery. The sewing material and stapling clips cause only a local tissue reaction within the anastomosis and have no known effect on the systemic inflammatory response and immune function after the operation [38].

CD64 index

CD64 is an FcRI receptor. It is a useful tool for diagnosing and assessing sepsis in patients and distinguishing between a septic or systemic inflammatory response [39–41]. Increased expression of CD64 molecules on neutrophils may be a good diagnostic indicator of the beginning of infection/sepsis [42]. It is almost not expressed in healthy human neutrophils. There has not been much research on the role of CD64 in early recognition of infectious complications in the postoperative period. Some trials have been done in orthopaedic and cardiovascular surgery, but not in abdominal surgery, which is in itself a contaminated or clean-contaminated procedure. Those studies have shown a greater increase in CD64 in patients who later developed an infectious complication [42]. When observing the neutrophil CD64 index, we found no significant differences between the two groups of patients (p = 0.44), but 24 h after surgery there was a considerable increase in CD64 index in both groups (Figure 1 I). The maximum mean CD64 index in the LAS group (after 24 h) was 1.24 and was on the threshold for sepsis (1.2), while the maximum mean index in the OCS group (after 24 h) was 1.42 and was above that threshold (1.2). This implies that there may be a greater release of bacteria into the bloodstream following open type surgery compared to laparoscopic surgery. However, we did not observe any differences in the occurrence of postsurgical infectious complications or sepsis in any group. The role of CD64 in the early postoperative period should be determined on a larger sample with observed postoperative infections, especially since its role might be questionable due to a variety of postoperative disturbances in the immune system.

Cytokine expression

Mean values of the observed cytokines IL-8, IL-10, IL-1b, IL-12p70 and TNF were slightly higher in the OCS group of patients 4 days after surgery, although the differences were not statistically significant (Table IV). Higher levels of IL-10 are consistent with higher levels of immunosuppression in open surgical procedures, since high IL-10 serum concentrations have profound immunosuppressive actions [43]. The IL-8 and TNF levels correlate with a higher acute inflammatory response in patients who underwent a conventional surgical procedure. However, those two cytokines may also be involved in the antitumor response, so their role may be more universal. We found no differences in IL-6 production between the groups (p = 0.68), even though surgical trauma induces a significant release of IL-6, which triggers an acute-phase response. This may be because the peak of IL-6 concentrations is present 3 h after surgery and it returns to preoperative values in a few days [12].

In conclusions, it seems that there are important advantages to minimally invasive surgery with regard to short-term clinical outcomes, which is consistent with other studies. Our patients had less blood loss, shorter duration of hospital stay and faster resumption of oral food intake after a minimally invasive procedure. Less tissue damage and lower surgical stress importantly contribute to faster recovery.

The clinical significance of immune disturbances following different surgical approaches has not yet been fully determined. No attempt to directly connect immunological parameters with clinical outcomes was attempted in our study, and there could be other factors contributing to clinical outcomes. Therefore, changes in observed immunological parameters may have more universal meaning. Further studies are needed to elucidate this topic.

Overall, our results show similar trends in post-surgical immunological disturbance between the two groups. Nonetheless, our data are of clinical interest – firstly, because we observed a divergent effect of different surgical approaches on the expression of HLA-DR on monocytes; and secondly, although our findings reflect a well-researched topic, they are the first results reported for Slovenia and are of great value in our local environment.

Conflict of interest

The authors declare no conflict of interest.