Figure 3.
Macrophage depletion alleviates anemia and ineffective erythropoiesis in mDia2 hematopoietic specific knockout mice. (A) Eight-week old littermate mice with indicated genotypes were retroorbitally injected with a single dose of clodronate liposomes (150 μl) or vehicle control (Phosphate buffered saline liposomes). Peripheral blood was collected two days after injection and the indicated red blood cell indices were measured using an automated hematology analyzer. (B) Wright-Giemsa stains of peripheral blood smears from indicated mice. Scale bars: 10 μm. (C-D) Quantification of populations I-VI from flow cytometric analysis of terminal erythropoiesis in bone marrow (C) and spleen (D) of indicated mice, as in Figure 1C and 1E. N = 5 in each group. (E) Quantification of spleen size of indicated mice (Left). Photographs of spleens from mDia2fl/flVav-Cre mice treated with vehicle control and clodronate liposomes (Right). N = 5 in each group.