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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Nature. 2014 Feb 16;507(7491):258–261. doi: 10.1038/nature12971

Extended Data Table 3.

TtAgo preferentially acquires ssDNA guides from plasmid DNA

DNA locus Size Copies per cell Total DNA per cell Reads aligned to DNA locus Normalized reads* Normalized reads* corrected for DNA per cell
E. coli K12 chromosome 4.64 Mb 1 4.64 Mb 23*106 1 1
pWUR702 5.6 kb 20-40 0.17 Mb 45*106 2 54
pRARE 4.7 kb 10-12 52 kb 2*106 0.1 8.8

Estimated relative quantities of guides complementary to plasmid and chromosome DNA per cell.

*

Reads are normalized against the number of reads mapped against the E. coli K12 chromosome.