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. Author manuscript; available in PMC: 2016 Jan 5.
Published in final edited form as: Circulation. 2006 Feb 13;113(7):1005–1014. doi: 10.1161/CIRCULATIONAHA.105.588954

Figure 1.

Figure 1

Stable lentiviral transduction of ES cells with the TF reporter gene. a, Schema of the TF reporter gene containing fusion of fluc-mrfp-ttk. The TF reporter gene was cloned into a self-inactivating lentiviral vector downstream from the ubiquitin promoter. The 3 fusion proteins are joined by a 14-amino acid (LENSHASAGYQAST) and 8-amino acid (TAGPGSAT) linker, respectively. b, FACS histograms of ES cells 48 hours after transduction with plasmid lipofectamine, electroporation, and lentivirus carrying the TF reporter gene. c, Control ES and ES-TF cells showed similar morphology and stem cell marker Oct4 expression on brightfield and fluorescence microscopy, respectively. DAPI staining is used as a nuclear marker. The stability of TF reporter gene expression was measured quantitatively by both (d) fluc enzyme assays and (e) mrfp FACS for 40 passages. LVLTR indicates lentivirus long-terminal repeat.