Abstract
Exogenous application of pokeweed antiviral protein (PAP), a ribosome-inhibiting protein found in the cell walls of Phytolacca americana (pokeweed), protects heterologous plants from viral infection. A cDNA clone for PAP was isolated and introduced into tobacco and potato plants by transformation with Agrobacterium tumefaciens. Transgenic plants that expressed either PAP or a double mutant derivative of PAP showed resistance to infection by different viruses. Resistance was effective against both mechanical and aphid transmission. Analysis of the vacuum infiltrate of leaves expressing PAP showed that it is enriched in the intercellular fluid. Analysis of resistance in transgenic plants suggests that PAP confers viral resistance by inhibiting an early event in infection. Previous methods for creating virus-resistant plants have been specific for a particular virus or closely related viruses. To protect plants against more than one virus, multiple genes must be introduced and expressed in a single transgenic line. Expression of PAP in transgenic plants offers the possibility of developing resistance to a broad spectrum of plant viruses by expression of a single gene.
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Selected References
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- A simple and general method for transferring genes into plants. Science. 1985 Mar 8;227(4691):1229–1231. doi: 10.1126/science.227.4691.1229. [DOI] [PubMed] [Google Scholar]
- Barbieri L., Aron G. M., Irvin J. D., Stirpe F. Purification and partial characterization of another form of the antiviral protein from the seeds of Phytolacca americana L. (pokeweed). Biochem J. 1982 Apr 1;203(1):55–59. doi: 10.1042/bj2030055. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Braun C. J., Hemenway C. L. Expression of Amino-Terminal Portions or Full-Length Viral Replicase Genes in Transgenic Plants Confers Resistance to Potato Virus X Infection. Plant Cell. 1992 Jun;4(6):735–744. doi: 10.1105/tpc.4.6.735. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Golemboski D. B., Lomonossoff G. P., Zaitlin M. Plants transformed with a tobacco mosaic virus nonstructural gene sequence are resistant to the virus. Proc Natl Acad Sci U S A. 1990 Aug;87(16):6311–6315. doi: 10.1073/pnas.87.16.6311. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Irvin J. D., Kelly T., Robertus J. D. Purification and properties of a second antiviral protein from Phytolacca americana which inactivates eukaryotic ribosomes. Arch Biochem Biophys. 1980 Apr 1;200(2):418–425. doi: 10.1016/0003-9861(80)90372-0. [DOI] [PubMed] [Google Scholar]
- Irvin J. D. Purification and partial characterization of the antiviral protein from Phytolacca americana which inhibits eukaryotic protein synthesis. Arch Biochem Biophys. 1975 Aug;169(2):522–528. doi: 10.1016/0003-9861(75)90195-2. [DOI] [PubMed] [Google Scholar]
- Kay R., Chan A., Daly M., McPherson J. Duplication of CaMV 35S Promoter Sequences Creates a Strong Enhancer for Plant Genes. Science. 1987 Jun 5;236(4806):1299–1302. doi: 10.1126/science.236.4806.1299. [DOI] [PubMed] [Google Scholar]
- Lawson C., Kaniewski W., Haley L., Rozman R., Newell C., Sanders P., Tumer N. E. Engineering resistance to mixed virus infection in a commercial potato cultivar: resistance to potato virus X and potato virus Y in transgenic Russet Burbank. Biotechnology (N Y) 1990 Feb;8(2):127–134. doi: 10.1038/nbt0290-127. [DOI] [PubMed] [Google Scholar]
- Lin Q., Chen Z. C., Antoniw J. F., White R. F. Isolation and characterization of a cDNA clone encoding the anti-viral protein from Phytolacca americana. Plant Mol Biol. 1991 Oct;17(4):609–614. doi: 10.1007/BF00037047. [DOI] [PubMed] [Google Scholar]
- Longstaff M., Brigneti G., Boccard F., Chapman S., Baulcombe D. Extreme resistance to potato virus X infection in plants expressing a modified component of the putative viral replicase. EMBO J. 1993 Feb;12(2):379–386. doi: 10.1002/j.1460-2075.1993.tb05669.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Owens R. A., Bruening G., Shepherd R. J. A possible mechanism for the inhibition of plant viruses by a peptide from Phytolacca americana. Virology. 1973 Nov;56(1):390–393. doi: 10.1016/0042-6822(73)90319-x. [DOI] [PubMed] [Google Scholar]
- Ready M. P., Brown D. T., Robertus J. D. Extracellular localization of pokeweed antiviral protein. Proc Natl Acad Sci U S A. 1986 Jul;83(14):5053–5056. doi: 10.1073/pnas.83.14.5053. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Richins R. D., Scholthof H. B., Shepherd R. J. Sequence of figwort mosaic virus DNA (caulimovirus group). Nucleic Acids Res. 1987 Oct 26;15(20):8451–8466. doi: 10.1093/nar/15.20.8451. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stirpe F., Barbieri L., Battelli M. G., Soria M., Lappi D. A. Ribosome-inactivating proteins from plants: present status and future prospects. Biotechnology (N Y) 1992 Apr;10(4):405–412. doi: 10.1038/nbt0492-405. [DOI] [PubMed] [Google Scholar]
- Taylor B. E., Irvin J. D. Depurination of plant ribosomes by pokeweed antiviral protein. FEBS Lett. 1990 Oct 29;273(1-2):144–146. doi: 10.1016/0014-5793(90)81070-5. [DOI] [PubMed] [Google Scholar]
- Vancanneyt G., Schmidt R., O'Connor-Sanchez A., Willmitzer L., Rocha-Sosa M. Construction of an intron-containing marker gene: splicing of the intron in transgenic plants and its use in monitoring early events in Agrobacterium-mediated plant transformation. Mol Gen Genet. 1990 Jan;220(2):245–250. doi: 10.1007/BF00260489. [DOI] [PubMed] [Google Scholar]
- Zarling J. M., Moran P. A., Haffar O., Sias J., Richman D. D., Spina C. A., Myers D. E., Kuebelbeck V., Ledbetter J. A., Uckun F. M. Inhibition of HIV replication by pokeweed antiviral protein targeted to CD4+ cells by monoclonal antibodies. Nature. 1990 Sep 6;347(6288):92–95. doi: 10.1038/347092a0. [DOI] [PubMed] [Google Scholar]