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Chiral phase HPLC analysis of HODE methyl ester derivatives. Panels A and B: resolution of 9RS-HODE and 13RS-HODE, respectively. Panel C: 9-HODE from wild-type tomato LOXA. Panel D: 9-HODE from tomato LOXA Ala564Gly. Panel E: 13-HODE from tomato LOXA Ala564Gly. A Diacel Chiralpak AD column (5μ, 25 × 0.46 cm) was eluted at 1 ml/min with hexane/methanol (100/2, by volume) with UV detection at 235 nm.
