Table 2.
PCR screening for five reproductive symbionts.
| Genus | Primer 5’-3’ | Tm°C | Product Size | Reference |
|---|---|---|---|---|
| Wolbachia | wspecF YATACCTATTCGAAGGGATAG |
55 °C | 438 bp | Werren and Windsor 2000 |
| wspecR AGCTTCGAGTGAAACCAATTC |
||||
| Spiroplasma | 63F_CG | 60 °C | 450 bp |
Mateos et al. 2006 Fukatsu and Nikoh 2000 |
| GCCTAATACATGCAAGTCGAACGG | ||||
| TKSSspR | ||||
| TAGCCGTGGCTTTCTGGTAA | ||||
| Arsenophonus | ArsF | 56 °C | 611 bp | Duron et al. 2008b |
| GGGTTGTAAAGTACTTTCAGTCGT | ||||
| ArsR5 | ||||
| CCCTAAGGCACGYYTYTATCTCTAA | ||||
| Rickettsia | 16SA1 | 55 °C | 200 bp | Fukatsu and Nikoh 2000 |
| AGAGTTTGATCTGGCTCAG | ||||
| Rick16SR | ||||
| CATCCATCAGCGATAAATCTTTC | ||||
| Cardinium | CLO-f1 | 56 °C | 466 bp | Gotoh et al. 2007 |
| GGAACCTTACCTGGGCTAGAATGTATT | ||||
| CLO-r1 | ||||
| GCCACTGTCTTCAAGCTCTACCAAC |