Abstract
The distribution of lactoferrin and glycoprotein in human bronchial glands has been studied by electron microscopy using an immunoperoxidase method to stain the former and a periodic acid-chromic acid-silver methenamine sequence for the latter, each applied to ultrathin Epon sections. The distribution of lactoferrin corresponds to that of lysozyme. Lactoferrin and lysozyme are both confined to serous acini where the granules show a variable pattern of staining. Some serous granules are filled uniformly with lactoferrin, some lack lactoferrin in a small central core or a thin peripheral rim, and some are completely devoid of lactoferrin. Glycoprotein is present in all mucous granules but only certain serous granules. The latter may be filled uniformly with glycoprotein or glycoprotein may form a thin peripheral coat about centrally located lactoferrin and lysozyme. An electron-dense central core found in some serous granules contains neither glycoprotein, lactoferrin, nor lysozyme.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bowes D., Corrin B. Ultrastructural immunocytochemical localisation of lysozyme in human bronchial glands. Thorax. 1977 Apr;32(2):163–170. doi: 10.1136/thx.32.2.163. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Erlandsen S. L., Parsons J. A., Taylor T. D. Ultrastructural immunocytochemical localization of lysozyme in the Paneth cells of man. J Histochem Cytochem. 1974 Jun;22(6):401–413. doi: 10.1177/22.6.401. [DOI] [PubMed] [Google Scholar]
- Klockars M., Reitamo S. Tissue distribution of lysozyme in man. J Histochem Cytochem. 1975 Dec;23(12):932–940. doi: 10.1177/23.12.1104708. [DOI] [PubMed] [Google Scholar]
- MCCARTHY C., REID L. INTRACELLULAR MUCOPOLYSACCHARIDES IN THE NORMAL HUMAN BRONCHIAL TREE. Q J Exp Physiol Cogn Med Sci. 1964 Jan;49:85–94. doi: 10.1113/expphysiol.1964.sp001709. [DOI] [PubMed] [Google Scholar]
- Mason D. Y., Taylor C. R. The distribution of muramidase (lysozyme) in human tissues. J Clin Pathol. 1975 Feb;28(2):124–132. doi: 10.1136/jcp.28.2.124. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Masson P. L., Heremans J. F., Prignot J. J., Wauters G. Immunohistochemical localization and bacteriostatic properties of an iron-binding protein from bronchial mucus. Thorax. 1966 Nov;21(6):538–544. doi: 10.1136/thx.21.6.538. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Meyrick B., Reid L. Ultrastructure of cells in the human bronchial submucosal glands. J Anat. 1970 Sep;107(Pt 2):281–299. [PMC free article] [PubMed] [Google Scholar]